PXD016833

PXD016833 is an original dataset announced via ProteomeXchange.

Title	Plasmodium condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission
Description	Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine the role of SMC2 and SMC4, the core subunits of condensin, during endomitosis in schizogony and endoreduplication in male gametogenesis. During early schizogony SMC2/SMC4 localize to a distinct focus, identified as the centromeres by NDC80 fluorescence and ChIP-seq analyses, but do not form condensin I or II complexes. In mature schizonts and during male gametogenesis there is a diffuse SMC2/SMC4 distribution on chromosomes and in the nucleus, and both condensin I and II complexes form at these stages. Knockdown of smc2 and smc4 gene expression revealed essential roles in parasite proliferation and transmission. The condensin core subunits (SMC2/SMC4) form different complexes and may have distinct functions at various stages of the parasite life cycle.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:12:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016833
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-19 05:35:07	ID requested
⏵ 1	2020-05-26 16:12:49	announced

Pandey R, Abel S, Boucher M, Wall RJ, Zeeshan M, Rea E, Freville A, Lu XM, Brady D, Daniel E, Stanway RR, Wheatley S, Batugedara G, Hollin T, Bottrill AR, Gupta D, Holder AA, Le Roch KG, Tewari R, Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission. Cell Rep, 30(6):1883-1897.e6(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Malaria, Plasmodium berghei, LC-MS, SCM2, SCM4, parasite, Condensin, Warwick-RTP

Dr Rita Tewari
contact affiliation	School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK
contact email	rita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016833
     1. Label: PRIDE project
     2. Name: Plasmodium condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission