PXD016824

PXD016824 is an original dataset announced via ProteomeXchange.

Title	Deer antler regenerative stem cell tissues LC-MS/MS
Description	As the only known mammalian organ that can fully and annually regenerate, deer antler has significant advantages over lower-order animal models when investigating the control of stem cell-based organ regeneration. Antler regeneration is known to be initiated and maintained by neural crest-derived stem cells in different states of activation. Antler stem cells can therefore be used as a model system to study the proteins and pathways involved in the maintenance of a stem cell niche and their activation and differentiation during organ formation. In the current study, the MSC markers CD73, CD90 and CD105 were examined within the antler tip. Label-free quantification was performed to investigate the protein profiles of antler stem cells under different stages of activation and included: dormant pedicle periosteum (DPP), antler growth center (GC), post-active stem cells from mid-beam antler periosteum (MAP), and deer facial periosteum (FP) as a control (n = 3 per group). PEAKS and IPA software were used to analyze the proteomic data. Our research confirmed the central role of stem cell activation in the development of this mammalian organ by localizing the MSC markers within the antler growth center. Label-free quantification revealed that the greatest number of unique proteins (eighty-seven) was found in the growth center tissue. There were only 12 proteins found with expression levels that significantly differed between DPP and FP. Protein profiles of these two groups indicated that antler stem cells may use similar mechanisms to maintain dormancy within a stem cell niche. The number of significantly regulated proteins between DPP, MAP and GC was 153. Among them, the majority were upregulated in the growth center. Activation of antler stem cells was associated with a number of biological processes and signaling pathways such as Hippo and canonical Wnt signaling. This work identifies the key canonical pathways, molecular/cellular functions and upstream regulators involved in mammal organ regeneration.
HostingRepository	iProX
AnnounceDate	2019-12-19
AnnouncementXML	Submission_2020-03-16_20:31:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Dong
SpeciesList	scientific name: Cervus elaphus; NCBI TaxID: 9860;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-18 21:13:42	ID requested
1	2019-12-18 21:16:01	announced
⏵ 2	2020-03-16 20:31:37	announced	2020-03-17: Update publication information.

Dong Z, Haines S, Coates D, Proteomic Profiling of Stem Cell Tissues during Regeneration of Deer Antler: A Model of Mammalian Organ Regeneration. J Proteome Res, 19(4):1760-1775(2020) [pubmed]

submitter keyword: Animal regeneration models, deer antler, neural crest, antler stem cell, label-free quantification

Dawn Coates
contact affiliation	Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, New Zealand
contact email	dawn.coates@otago.ac.nz
lab head
Zhen Dong
contact affiliation	Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, New Zealand
contact email	donzh369@student.otago.ac.nz
dataset submitter

iProX dataset URI