PXD016811

PXD016811 is an original dataset announced via ProteomeXchange.

Title	sRNA scr5239 involved in important feedback loop of S. coelicolor central metabolism
Description	The project is focused on the highly conserved sRNA scr5329 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them the Phosphoenolpyruvate-Carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 promotes its degradation on the post-transcriptional level. The expression itself is dependent on the global transcriptional regulator DasR, which is N-acetylglucosamine-responsive thereby creating a feedback regulation. By post-transcriptional regulation of PEPCK and likely more targets, scr5239 adds an additional layer to the DasR regulatory network, providing a tool to control the metabolism in dependency to the carbon source.
HostingRepository	PRIDE
AnnounceDate	2020-01-28
AnnouncementXML	Submission_2020-01-27_23:56:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Ossipova
SpeciesList	scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-18 01:26:08	ID requested
⏵ 1	2020-01-27 23:56:51	announced

Dataset with its publication pending

submitter keyword: sRNA, Streptomyces, DasR, carbon metabolism

Beatix Suess
contact affiliation	Synthetic Genetic Circuits, Department of Biology, Technical University Darmstadt, Darmstadt, Germany
contact email	bsuess@bio.tu-darmstadt.de
lab head
Elena Ossipova
contact affiliation	Karolinska Institute
contact email	Elena.Ossipova@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016811

PRIDE project URI

• PRIDE
  1. PXD016811
     1. Label: PRIDE project
     2. Name: sRNA scr5239 involved in important feedback loop of S. coelicolor central metabolism