PXD016809

PXD016809 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics analysis to identify PPP2R5D substrates
Description	The purpose of this project is to identify the substrates that are specific for B'delta regulatory subunit. For this purpose, we have generated two doxycycline-inducible stable cell lines (OE and RE) that express B'delta. In the OE cell lines, B'delta is overexpressed without downregulation of the endogenous regulatory subunits. On the other hand, in the RE cell lines, B'delta is overexpressed concurrently with a downregulation of the endogenous regulatory subunits. To stimulate protein phosphorylation, we used isoproterenol, a beta-adrenergic agonist that is known to induce the PKA-mediated phosphorylation and activation of the B'd.
HostingRepository	PRIDE
AnnounceDate	2020-04-17
AnnouncementXML	Submission_2020-04-16_22:39:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Herring
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-18 01:25:49	ID requested
⏵ 1	2020-04-16 22:39:46	announced

Jong CJ, Merrill RA, Wilkerson EM, Herring LE, Graves LM, Strack S, holoenzyme. J Biol Chem, 295(17):5654-5668(2020) [pubmed]

submitter keyword: phosphoproteomics, PPP2R5D

Stephan Strack
contact affiliation	University of Iowa
contact email	stefan-strack@uiowa.edu
lab head
Laura Herring
contact affiliation	UNC-Chapel Hill
contact email	laura_herring@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016809

PRIDE project URI

• PRIDE
  1. PXD016809
     1. Label: PRIDE project
     2. Name: Phosphoproteomics analysis to identify PPP2R5D substrates