PXD016803

PXD016803 is an original dataset announced via ProteomeXchange.

Title	Global characterization of miR-574-5p decoy to CUGBP1 in human lung cancer using a mass spectrometry proteomics approach
Description	We aimed to characterize decoy to the RNA-binding protein CUG-RNA binding protein 1 (CUGBP1 mechanism in A549 lung cancer cells. We identified several new canonical targets of CUGBP1 but those were not regulated by miR-574-5p via the decoy mechanism. This can be explained by the localization of CUGBP1 and miR-574-5p in the nucleus, where CUGBP1 regulates alternative splicing. Next, we analyzed the 3’UTRs of potential targets and found that the decoy-dependent regulation of mPGES-1 splicing is unique. Therefore, we postulate that in A549 cells mPGES-1 is the only protein regulated by the decoy mechanism of CUGBP1 and miR-574-5p which suggests that the decoy mechanism allows the specific regulation of the expression of distinct targets.
HostingRepository	PRIDE
AnnounceDate	2020-03-03
AnnouncementXML	Submission_2020-04-02_22:59:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Ossipova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-17 06:11:05	ID requested
1	2020-03-03 02:17:37	announced
⏵ 2	2020-04-02 22:59:13	announced	2020-04-03: Updated publication reference for PubMed record(s): 32231562.

Emmerich AC, Wellstein J, Ossipova E, Baumann I, Lengqvist J, Kultima K, Jakobsson PJ, Steinhilber D, Saul MJ, Proteomics-Based Characterization of miR-574-5p Decoy to CUGBP1 Suggests Specificity for mPGES-1 Regulation in Human Lung Cancer Cells. Front Pharmacol, 11():196(2020) [pubmed]

submitter keyword: human, lung cancer, miR-574-5p, CUGBP1, mPGES-1

Meike J. Saul
contact affiliation	Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany Institute of Pharmaceutical Chemistry, Goethe Universität Frankfurt, 60438 Frankfurt/M., Germany
contact email	saul@bio.tu-darmstadt.de
lab head
Elena Ossipova
contact affiliation	Karolinska Institute
contact email	Elena.Ossipova@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016803

PRIDE project URI

• PRIDE
  1. PXD016803
     1. Label: PRIDE project
     2. Name: Global characterization of miR-574-5p decoy to CUGBP1 in human lung cancer using a mass spectrometry proteomics approach