PXD016793 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics analysis of rat liver after disulfiram treatment |
Description | Obesity has become a global health concern. A molecule that safely prevents and/or treats obesity is urgently needed. Here, we show that long-term diet supplementation of disulfiram (DSF, Antabuse®), an FDA-approved drug, abrogated the adverse impact of an obesogenic diet on insulin responsiveness, while mitigating liver steatosis, cardiovascular remodeling, and pancreatic islet hypertrophy in mice. Additionally, DSF treatment of already obese male and female mice reversed weight gain and alleviated metabolic dysfunctions. Loss of fat tissue and increase in liver fenestrations were also observed in rats on DSF. Transcriptomics and proteomics analyses of mouse and rat livers unveiled comparable signatures, as revealed by pathways associated with lipid and energy metabolism, redox, and detoxification. Mechanistically, markers of hepatic oxidative stress were abrogated in DSF-treated mice through activation of ALDH2 and GST activities in vivo and stimulation of autophagic flux in vitro. Thus, repurposing DSF may represent a new strategy to combat obesity and reverse the associated metabolic disorders in human. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-15 |
AnnouncementXML | Submission_2020-08-11_03:15:14.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mark Larance |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-17 02:03:54 | ID requested | |
1 | 2020-05-15 00:00:59 | announced | |
⏵ 2 | 2020-08-11 03:15:15 | announced | 2020-08-11: Updated publication reference for PubMed record(s): 32714562. |
Publication List
Bernier M, Harney D, Koay YC, Diaz A, Singh A, Wahl D, Pulpitel T, Ali A, Guiterrez V, Mitchell SJ, Kim EY, Mach J, Price NL, Aon MA, LeCouteur DG, Cogger VC, Fernandez-Hernando C, O'Sullivan J, Larance M, Cuervo AM, de Cabo R, Elucidating the mechanisms by which disulfiram protects against obesity and metabolic syndrome. NPJ Aging Mech Dis, 6():8(2020) [pubmed] |
Keyword List
submitter keyword: disulfiram, rat, liver, metabolism, high-fat, obesity |
Contact List
Mark Larance |
contact affiliation | University of Sydney |
contact email | mark.larance@sydney.edu.au |
lab head | |
Mark Larance |
contact affiliation | The University of Sydney |
contact email | mark.larance@sydney.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016793
- Label: PRIDE project
- Name: Proteomics analysis of rat liver after disulfiram treatment