PXD016787

PXD016787 is an original dataset announced via ProteomeXchange.

Title	Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics
Description	A differential lysis approach was developed with different strength of lysis buffers (urea and/or sodium dodecyl sulfate) and applied on human stool samples to separate different microbial species to increase the depth of metaproteomic measurements. We showed that the lysates dominating by proteins from Gram-positive (G+) or negative bacteria (G-) can be achieved by differential lysis
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:30.792.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiaqin Wang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-17 02:03:13	ID requested
1	2023-03-27 04:01:24	announced
⏵ 2	2023-11-14 08:44:31	announced	2023-11-14: Updated project metadata.

Wang J, Zhang X, Li L, Ning Z, Mayne J, Schmitt-Ulms C, Walker K, Cheng K, Figeys D, Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics. Anal Chem, 92(7):5379-5386(2020) [pubmed]

submitter keyword: Human,LC-MSMS, Gut microbiome

Daniel Figeys
contact affiliation	BMI, Faculty of Medicine, University of Ottawa
contact email	dfigeys@uottawa.ca
lab head
Jiaqin Wang
contact affiliation	Ottawa Institute of Systems Biology and Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON K1H 8M5, Canada
contact email	wangjq_njnu@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD016787

PRIDE project URI

• PRIDE
  1. PXD016787
     1. Label: PRIDE project
     2. Name: Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics