PXD016782

PXD016782 is an original dataset announced via ProteomeXchange.

Title	Digestion of a variety of synthetic peptides by proteasomes
Description	Proteasomes are large multi-subunit enzymes that act as the main producers of antigenic peptides presented at the cell surface to CD8+ T cells. They can simply cut proteins or recombine their fragments thereby generating novel sequences, i.e. spliced peptides via a process called proteasome-catalysed peptide splicing (PCPS). In order to gain more insight into these processes, we here perform in vitro digestions of 55 synthetic polypeptide substrates by proteasome isoforms followed by LC-MS/MS measurements using different Mass Spectrometers.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:57.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Artem Mansurkhodzhaev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap XL; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-16 23:50:34	ID requested
1	2020-04-14 02:22:55	announced
2	2020-06-30 04:41:51	announced	2020-06-30: Updated publication reference for PubMed record(s): 32415162.
⏵ 3	2024-10-22 05:01:59	announced	2024-10-22: Updated project metadata.

10.1038/s41597-020-0487-6;

Specht G, Roetschke HP, Mansurkhodzhaev A, Henklein P, Textoris-Taube K, Urlaub H, Mishto M, Liepe J, Large database for the analysis and prediction of spliced and non-spliced peptide generation by proteasomes. Sci Data, 7(1):146(2020) [pubmed]

submitter keyword: Synthetic peptides, proteasome, digestion, LC-MS/MS

Juliane Liepe
contact affiliation	Head of research group Quantitative and Systems Biology; Max Planck Institute for Biophysical Chemistry; Goettingen;Germany
contact email	juliane.liepe@mpibpc.mpg.de
lab head
Artem Mansurkhodzhaev
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	artem.mansurkhodzhaev@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016782

PRIDE project URI

• PRIDE
  1. PXD016782
     1. Label: PRIDE project
     2. Name: Digestion of a variety of synthetic peptides by proteasomes