PXD016770
PXD016770 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Generation of aggregates of alpha-lactalbumin by UV-B light exposure |
| Description | Whey proteins are widely used as ingredients in the form of aggregates to obtain certain functionalities in food applications. The aim of this study was to understand how UV illumination generates aggregates of ?-lactalbumin (?-LA) as an alternative to heat treatments traditionally used for industrial production of protein aggregates. Absorption of UV light by ?-LA caused cleavage of disulfide bonds and release of thiol groups, which resulted in primarily disulfide-mediated aggregation. This process mediated efficient aggregation with up to 98% monomer conversion into aggregates through formation of intermolecular disulfide bonds, while only minor levels of nonreducible cross-links were observed. SDS-PAGE analysis revealed that illumination led to formation of dimeric, trimeric, and oligomeric forms of ?-LA. LC-MS/MS analysis showed that all of the four native disulfide bonds in ?-LA were cleaved by UV illumination but to different extents, and the extent of cleavage was found to be higher in the absence of calcium. Seventeen different non-native disulfides were formed after 24 h of UV illumination. Two dityrosine bonds were identified (Tyr103-Tyr103 and Tyr36-Tyr103) alongside ditryptophan (Trp118-Trp118) and tyrosine-tryptophan (Tyr50-Trp60) cross-links. In addition, Trp60, Trp118, Cys73, Cys91, Cys120, Phe80, Met90, His68, and His107 were found to be oxidized up to 12% as compared to a nonilluminated control. Our work illustrates that light exposure can be used for generation of ?-LA aggregates, but optimization of the illumination conditions is required to reduce oxidative damage to Trp, Cys, Phe, Met, and His residues. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-05-22 |
| AnnouncementXML | Submission_2020-05-22_02:51:11.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kasper Engholm-Keller |
| SpeciesList | scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913; |
| ModificationList | Oxidation; Dioxidation; Trioxidation; Cys->Dha; Trp->Kynurenin; Homocysteic_acid; Dethiomethyl; Deamidated; 3'-(3'-L-tyrosinyl)-L-tyrosine; L-cystine (cross-link) |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-12-16 01:54:57 | ID requested | |
| ⏵ 1 | 2020-05-22 02:51:12 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: alpha lactalbumin, UV, aggregation, disulfide, modification, whey, Illumination |
Contact List
| Marianne Nissen Lund | |
|---|---|
| contact affiliation | Department of Food Science, University of Copenhagen |
| contact email | mnl@food.ku.dk |
| lab head | |
| Kasper Engholm-Keller | |
| contact affiliation | University of Copenhagen |
| contact email | kasperengholmkeller@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084695/ |
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