PXD016760

PXD016760 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the shell organic matrix of Spondylus Gaederopus
Description	This work reports a comprehensive and integrated microstructural, biochemical and proteomics study on the shell matrix of Spondylus gaederopus, the Mediterranean thorny oyster. We investigate the skeletal matrix proteins which are involved in biomineralization and compare the identified Spondylus sequences with other shell proteins, that are publicly available in databases. Using high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS) we characterized several shell protein fractions, isolated by different bleaching treatments. We identified six shell proteins, which also displayed features and domains typically found in biomineralized tissues, including the prevalence of intrinsically disordered regions. However, many reconstructed peptide sequences (de novo) could not be matched to any known shell proteins and we suggest that these probably represent lineage-specific sequences. The proteomic data implies that Spondylus may have evolved a distinct molecular toolkit for biomineralization. Using high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS) we characterized several shell protein fractions, isolated by different bleaching treatments. Six shell proteins were identified, which displayed features and domains typically found in biomineralized tissues, including the prevalence of intrinsically disordered regions. However, most of the reconstructed peptide sequences (de novo) could not be matched to any known shell proteins and probably represent lineage-specific sequences.
HostingRepository	PRIDE
AnnounceDate	2020-03-31
AnnouncementXML	Submission_2020-03-31_01:31:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jorune Sakalauskaite
SpeciesList	scientific name: Spondylus gaederopus; NCBI TaxID: 235612;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-16 01:36:26	ID requested
⏵ 1	2020-03-31 01:31:29	announced

Sakalauskaite J, Plasseraud L, Thomas J, Alb, é, ric M, Thoury M, Perrin J, Jamme F, Broussard C, Demarchi B, Marin F, The shell matrix of the european thorny oyster, Spondylus gaederopus: microstructural and molecular characterization. J Struct Biol, 211(1):107497(2020) [pubmed]

submitter keyword: Shell proteins, biomineralization, mollusc shells, proteomics

Frédéric Marin
contact affiliation	UMR CNRS 6282 Biogéosciences, University of Burgundy-Franche-Comté, 6 Boulevard Gabriel, 21000 Dijon, France
contact email	frederic.marin@u-bourgogne.fr
lab head
Jorune Sakalauskaite
contact affiliation	University of Turin
contact email	jorune.sakalauskaite@unito.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016760
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the shell organic matrix of Spondylus Gaederopus