PXD016759

PXD016759 is an original dataset announced via ProteomeXchange.

Title	Triple SILAC based quantitative MS approach to determine Withaferin A regulated proteins in prostate cancer
Description	Withaferin A (WA) is a lactone extracted from Withania somnifera commonly known as Ashwagandha. WA has several therapeutic benefits. The current study was aimed to identify biomarkers that could be targeted by WA in prostate cancer (PCA) cells. We have used SILAC approach to identify WA-regulated proteins at 4 h and 24 time points in three PCA cell lines such as LNCaP, 22Rv1 and DU-145. Ontology prediction suggested that WA treatment can upregulate stress-responsive pathways and shutdown translation and cell metabolism to conserve energy. The cytoprotective stress granule (SG) protein G3BP1 showed upregulation in all the three tested cell lines in response to WA treatment, and subsequently, SGs formed at a higher rate in the WA treated cells. Knockdown (KD) of G3BP1 blocked WA-induced SG formation and reduced the cell survival. We speculate that the activation of G3BP1 and the formation of SGs might constitute a mechanism by which PCA cells induce cell protection after WA- treatment. Knock down of SG proteins such as G3BP1 could help to evade the cytoprotective effects of WA and to assist in the sensitization of cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:24:05.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Syam Prakash Somasekharan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-16 01:36:26	ID requested
1	2021-07-20 21:51:38	announced
⏵ 2	2024-10-22 05:24:05	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mass Spectrometry, Triple SILAC, DU-145, Stress granule, G3BP1,Prostate cancer, Withaferin A, LNCaP, 22Rv1

Syam Prakash Somasekharan
contact affiliation	Vancouver Prostate Centre, Vancouver, Canada
contact email	syamprakash@gmail.com
lab head
Syam Prakash Somasekharan
contact affiliation	Vancouver Prostate Centre
contact email	syamprakash@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016759
     1. Label: PRIDE project
     2. Name: Triple SILAC based quantitative MS approach to determine Withaferin A regulated proteins in prostate cancer