PXD016754

PXD016754 is an original dataset announced via ProteomeXchange.

Title	Label-free proteomics of two bacterial strains grown on hydrocarbon
Description	Two bacterial strains isolated from Chinese oilfield, Dietzia sp. DQ12-45-1b and P. stutzeri SLG510A3-8, were found having synergistic effect on hydrocarbon biodegradation. To investigate the synergy mechanism between the two strains, they were cultivated separately or individually in a two-chamber bioreactor containing the mineral medium supplemented with hexadecane, and were harvested at the later exponential phase for the label-free proteomics analysis on LC-LTQ-Orbitrap MS. Therefore, four groups of proteome profiles were obtained, including the one from Dietzia sp. monoculture, the one from P. stutzeri monoculture, the one from Dietzia sp. in the co-culture system, and the one from P. stutzeri in the co-culture system. The comparative proteomics analysis between the mono- and co-cultivated Dietzia sp. showed that a total of 222 Dietzia sp. proteins had significantly differential expression in the two groups. For P. stutzeri, it was statistically found that a total of 198 P. stutzeri proteins in P. stutzeri mono- and co-culture systems had significantly differential expression. Afterward, by integrating the proteomics data with our in silico data, the synergistic hexadecane degradation was found to be attributed to the cross-feeding and intermediate-induced metabolic regulation between the two strains.
HostingRepository	iProX
AnnounceDate	2019-12-15
AnnouncementXML	Submission_2023-03-01_19:25:20.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bing Hu
SpeciesList	scientific name: Dietzia sp. DQ12-45-1b; NCBI TaxID: 912801; scientific name: Pseudomonas stutzeri; NCBI TaxID: 316;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-15 18:24:12	ID requested
1	2019-12-15 18:26:45	announced
⏵ 2	2023-03-01 19:25:22	announced	2023-03-02: Update publication information.

Hu B, Wang M, Geng S, Wen L, Wu M, Nie Y, Tang YQ, Wu XL, sp. Strain DQ12-45-1b. Appl Environ Microbiol, 86(8):(2020) [pubmed]

submitter keyword: microbial interaction

Bing Hu
contact affiliation	Beijing Institute of Technology
contact email	binghu319@bit.edu.cn
lab head
Bing Hu
contact affiliation	Beijing Institute of Technology
contact email	binghu319@bit.edu.cn
dataset submitter

iProX dataset URI