PXD016746

PXD016746 is an original dataset announced via ProteomeXchange.

Title	Coordination and assembly of protein complexes encoded across mitochondrial and nuclear genomes is assisted by mtCLPP in Arabidopsis thaliana.
Description	Protein homeostasis in eukaryotic organelles and their progenitor prokaryotes is regulated by a series of proteases including the caseinolytic protease (CLPP). CLPP has essential roles in chloroplast biogenesis and maintenance, but the significance of the plant mitochondrial CLPP remains unknown and factors that aid coordination of nuclear and mitochondrial encoded subunits for complex assembly in mitochondria await discovery. We generated knock-out lines of the single copy mitochondrial CLP protease subunit, CLPP2, in Arabidopsis thaliana. Mutants have higher abundance of transcripts from mitochondrial genes encoding OXPHOS protein complexes, while transcripts for nuclear genes encoding other subunits of the same complexes showed no change in transcript abundance. In contrast, the protein abundance of specific nuclear-encoded subunits in OXPHOS complexes I and V increased in knockouts, without accumulation of mitochondrial-encoded counterparts in the same complex. Protein complexes mainly or entirely encoded in the nucleus were unaffected. Analysis of protein import, assembly and function of Complex I revealed that while function was retained, protein homeostasis was disrupted through slower assembly, leading to accumulation of soluble subcomplexes of nuclear-encoded subunits. Therefore, CLPP2 contributes to the mitochondrial protein degradation network through supporting coordination and assembly of protein complexes encoded across mitochondrial and nuclear genomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:22.985.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakob Petereit
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-13 08:24:00	ID requested
1	2020-09-13 22:24:27	announced
⏵ 2	2024-10-22 05:12:23	announced	2024-10-22: Updated project metadata.

Petereit J, Duncan O, Murcha MW, Fenske R, Cincu E, Cahn J, Pru, ž, insk, á A, Ivanova A, Kollipara L, Wortelkamp S, Sickmann A, Lee J, Lister R, Millar AH, Huang S, Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes. Plant Physiol, 184(1):148-164(2020) [pubmed]

10.1104/pp.20.00136;

submitter keyword: CLPP, protease,mitochondrial genome,nuclear genome,Complex I

Andrew Harvey Millar
contact affiliation	ARC Centre of Excellence in Plant Energy Biology at The University of Western Australia
contact email	harvey.millar@uwa.edu.au
lab head
Jakob Petereit
contact affiliation	ARC Centre of Excellence in Plant Energy Biology
contact email	petinho86@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016746
     1. Label: PRIDE project
     2. Name: Coordination and assembly of protein complexes encoded across mitochondrial and nuclear genomes is assisted by mtCLPP in Arabidopsis thaliana.