PXD016740

PXD016740 is an original dataset announced via ProteomeXchange.

Title	Impact of an antifungal insect defensin on the proteome of the phytopathogenic fungus Botrytis cinerea
Description	ETD151, an analogue of the antifungal insect defensin heliomicin, is an antifungal peptide active against yeasts and filamentous fungi. In order to decipher the mechanisms underlying its molecular action on the phytopathogenic fungus Botrytis cinerea, a necrotrophic pathogen responsible for gray mold disease, we investigated the changes in three-day old mycelia upon treatment with different concentrations of ETD151. Optical and fluorescent microscopies were used prior to establishing the peptide/protein profiles through two mass spectrometry approaches: MALDI profiling, to generate molecular mass fingerprints as peptide signatures, and a gel-free bottom-up proteomics approach. Our results show that a concentration of ETD151 above the half-maximal inhibitory concentration can alter the integrity of the mycelial structure of B. cinerea. Furthermore, reproducible modifications of the peptide/protein composition were demonstrated in the presence of ETD151 within a 1.5-16 kDa mass range. After the robustness of LC-ESI-MS/MS analysis on B. cinerea mycelial extracts was confirmed, our analyses highlighted 340 significantly modulated proteins upon treatment with ETD151 within a 4.8- 466 kDa mass range. Finally, data mapping on KEGG pathways revealed the molecular impact of ETD151 on at least six pathways. In particular, for the effect on oxidative phosphorylation, we clearly demonstrated that ETD151 does not interact directly with the mitochondrial respiratory chain.
HostingRepository	PRIDE
AnnounceDate	2020-01-29
AnnouncementXML	Submission_2020-01-29_06:53:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien VOISIN
SpeciesList	scientific name: Botrytis cinerea; NCBI TaxID: 40559;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-13 08:18:36	ID requested
⏵ 1	2020-01-29 06:53:09	announced

Aumer T, Voisin SN, Knobloch T, Landon C, Bulet P, . J Proteome Res, 19(3):1131-1146(2020) [pubmed]

submitter keyword: Insect defensin, antifungal defensin, Botrytis cinerea, proteomics, fungal infection, heliomicin, mechanism of action

Philippe Bulet
contact affiliation	CR Université Grenoble Alpes, Institute for Advanced Biosciences, Inserm U1209, CNRS UMR 5309, Grenoble, France
contact email	philippe.bulet@univ-grenoble-alpes.fr
lab head
Sebastien VOISIN
contact affiliation	Biopark Archamps
contact email	Sebastien.VOISIN@biopark-archamps.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016740
     1. Label: PRIDE project
     2. Name: Impact of an antifungal insect defensin on the proteome of the phytopathogenic fungus Botrytis cinerea