PXD016726

PXD016726 is an original dataset announced via ProteomeXchange.

Title	Characterization of Macrophage Galactose-type Lectin (MGL) ligands in colorectal cancer cell lines
Description	Background The Ca2+-dependent C-type lectin receptor Macrophage Galactose-type Lectin (MGL) is highly expressed by tolerogenic dendritic cells (DC) and macrophages. MGL exhibits a high binding specificity for terminal alpha- and beta-linked GalNAc residues found in Tn, sTn and LacdiNAc antigens. These glycan epitopes are often overexpressed in colorectal cancer (CRC), and, as such, MGL can be used to discriminate tumor from the corresponding healthy tissues. Moreover, the high expression of MGL ligands is associated with poor disease-free survival in stage III of CRC tumors. Nonetheless, the glycoproteins expressed by tumor cells that are recognized by MGL have hitherto remained elusive. Methods Using a panel of three CRC cell lines (HCT116, HT29 and LS174T), recapitulating CRC diversity, we performed FACS staining and pull-down assays using a recombinant soluble form of MGL (and a mutant MGL as control) combined with mass spectrometry-based (glyco)proteomics. Results HCT116 and HT29, but not LS174T, are high MGL-binding CRC cell lines. On these cells, the major cell surface binding proteins are receptors (e.g. MET, PTK7, SORL1, PTPRF) and integrins (ITGB1, ITGA3). From these proteins, several N- and/or O-glycopeptides were identified, of which some carried either a LacdiNAc or Tn epitope.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-01-09_07:00:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yassene Mohammed
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-12 05:37:28	ID requested
⏵ 1	2020-01-09 07:00:06	announced

Sahasrabudhe NM, Lenos K, van der Horst JC, Rodr, í, guez E, van Vliet SJ, Oncogenic BRAFV600E drives expression of MGL ligands in the colorectal cancer cell line HT29 through N-acetylgalactosamine-transferase 3. Biol Chem, 399(7):649-659(2018) [pubmed]

submitter keyword: : Glycoproteomics

C-type lectin

colorectal cancer

LacdiNAc

Tn antigen

Paul J. Hensbergena
contact affiliation	Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands
contact email	P.J.Hensbergen@lumc.nl
lab head
Yassene Mohammed
contact affiliation	Leiden University Medcial Center
contact email	yassene@proteincentre.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016726
     1. Label: PRIDE project
     2. Name: Characterization of Macrophage Galactose-type Lectin (MGL) ligands in colorectal cancer cell lines