PXD016725

PXD016725 is an original dataset announced via ProteomeXchange.

Title	ADCK4 knockout Human podocyte iTRAQ
Description	Although identification of single gene mutations associated with SRNS have yielded insights into pathogenic mechanisms and localized its pathogenesis to glomerular podocytes, the disease mechanisms of SRNS remain obscure. ADCK4 mutations usually manifest as steroid-resistant nephrotic syndrome, and cause coenzyme Q10 (CoQ10) deficiency.The reduced form of CoQ (QH2) plays a role as a potent lipid-soluble antioxidant, scavenging free radicals and preventing lipid peroxidative damage. Although ADCK4 KO in itself did not affect the viability of cultured podocytes, we examined cell viability upon arachidonic acid (AA) treatment because CoQ-deficient yeast mutants were found to be more sensitive to polyunsaturated fatty acids such as AA, which are prone to autoxidation and breakdown into toxic products. To comprehensively understand the molecular changes induced by the KO of ADCK4, we performed proteomic analysis and quantified protein abundance changes by MS-based proteomics using isobaric tag for relative and absolute quantification (iTRAQ) in podocytes with and without AA treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:59.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SEYOUNG YU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-12 05:36:59	ID requested
1	2021-09-08 17:05:52	announced
⏵ 2	2024-10-22 05:27:07	announced	2024-10-22: Updated project metadata.

10.1681/asn.2019070756;

Widmeier E, Yu S, Nag A, Chung YW, Nakayama M, Fern, á, ndez-Del-R, í, o L, Hugo H, Schapiro D, Buerger F, Choi WI, Helmst, ä, dter M, Kim JW, Ryu JH, Lee MG, Clarke CF, Hildebrandt F, Gee HY, ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment. J Am Soc Nephrol, 31(6):1191-1211(2020) [pubmed]

ProteomeXchange project tag: Kidney Urine (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Mitochondria (HPP)

submitter keyword: Human, Arachidonic acid, ADCK4, iTRAQ, Podocyte

Heon Yung Gee
contact affiliation	Department of Pharmacology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Korea
contact email	hygee@yuhs.ac
lab head
SEYOUNG YU
contact affiliation	Department of Pharmacology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Korea
contact email	goldsy420@yuhs.ac
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD016725

PRIDE project URI

• PRIDE
  1. PXD016725
     1. Label: PRIDE project
     2. Name: ADCK4 knockout Human podocyte iTRAQ