PXD016714

PXD016714 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of KARS knockout zebrafish larvae
Description	KARS encodes for both mitochondrial and cytoplasmic lysyl-tRNA synthetase, which is necessary for protein synthesis. KARS mutations have been reported to be involved in hearing loss, vision disorder, neuropathology, etc. However, there is no relevant animal experimental research in vivo so far. Here, we developed a zebrafish KARS null mutant line, offering a model for diseases due to KARS mutations. Zebrafish embryos with different genotypes (homozygous or wild type) were separately collected for each biological replicate at 5 days post fertilization (dpf). Proteomics of three biological replicates for the two groups was performed and analyzed. Comparisons between these two groups allow identification of enriched proteins and pathways in KARS mutants, which may underlie phenotypes of KARS deletion. Our dataset provides a valuable resource for understanding the pathology and molecular mechanisms of KARS deficiency.
HostingRepository	iProX
AnnounceDate	2019-12-12
AnnouncementXML	Submission_2019-12-12_00:07:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiping Wang
SpeciesList	scientific name: Danio rerio; NCBI TaxID: 7955;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-12 00:04:39	ID requested
⏵ 1	2019-12-12 00:13:31	announced

Dataset with its publication pending

submitter keyword: zebrafish larvea, KARS， knockout, iTRAQ，PRM

Jiping Wang
contact affiliation	Otolaryngology Institute of Shanghai Jiao Tong University
contact email	tony20040135@aliyun.com
lab head
Jiping Wang
contact affiliation	Otolaryngology Institute of Shanghai Jiao Tong University
contact email	tony20040135@aliyun.com
dataset submitter

iProX dataset URI