PXD016687

PXD016687 is an original dataset announced via ProteomeXchange.

Title	BioID of Plasmodium falciparum rhophtry-neck associated protein (PfAIP) using BirA* fusion-protein
Description	Apicomplexan parasites contain rhoptries, which are specialized secretory organelles that coordinate host cell invasion. During the process of invasion, rhoptries secrete their contents to facilitate interaction with, and entry into, the host cell. Here we report the crystal structure of the rhoptry protein Armadillo Repeats-Only (ARO) from the human malaria parasite, Plasmodium falciparum (PfARO). The structure of PfARO is comprised of five tandem Armadillo-like (ARM) repeats, with adjacent ARM repeats stacked in a head-to-tail orientation resulting in PfARO adopting an elongated curved shape. Interestingly, the concave face of PfARO contains two distinct patches of highly conserved residues that appear to play an important role in protein-protein interaction. We functionally characterized the P. falciparum homologue of ARO interacting protein (PfAIP) and demonstrate that it localizes to the rhoptries. We show that conditional mislocalization of PfAIP leads to deficient red blood cell invasion. Guided by the structure, we identified mutations of PfARO that lead to mislocalization of PfAIP. Using proximity-based biotinylation we probe into PfAIP interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:34:26.406.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016687
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Strauss
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	orbitrap; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-11 01:33:30	ID requested
⏵ 1	2024-10-07 10:34:27	announced

Geiger M, Brown C, Wichers JS, Strauss J, Lill A, Thuenauer R, Liffner B, Wilcke L, Lemcke S, Heincke D, Pazicky S, Bachmann A, L, ö, w C, Wilson DW, Filarsky M, Burda PC, Zhang K, Junop M, Gilberger TW, Structural Insights Into PfARO and Characterization of its Interaction With PfAIP. J Mol Biol, 432(4):878-896(2020) [pubmed]

10.1016/j.jmb.2019.12.024;

10.6019/PXD016687;

curator keyword: Biomedical

submitter keyword: Malaria, invasion, proximity-based biotinylation, rhoptries, Plasmodium

Tim Gilberger
contact affiliation	CSSB Centre for Structural Systems Biology c/o Deutsches Elektronen-Synchrotron DESY Notkestraße 85, Building 15 D-22607 Hamburg
contact email	tim.gilberger@cssb-hamburg.de
lab head
Jan Strauss
contact affiliation	Centre for Structural Systems Biology (CSSB), DESY, and European Molecular Biology Laboratory Hamburg
contact email	jan.strauss@cssb-hamburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016687

PRIDE project URI

• PRIDE
  1. PXD016687
     1. Label: PRIDE project
     2. Name: BioID of Plasmodium falciparum rhophtry-neck associated protein (PfAIP) using BirA* fusion-protein