PXD016681 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1 |
Description | We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or FLAG-tagged kinases immunopurified from transiently transfected HEK293 cells |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:59:23.163.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jack-Ansgar Bruun |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-10 07:25:27 | ID requested | |
1 | 2020-01-23 00:17:29 | announced | |
⏵ 2 | 2024-10-22 04:59:25 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/jbc.ra119.010068; |
Shrestha BK, Skytte Rasmussen M, Abudu YP, Bruun JA, Larsen KB, Alemu EA, Sj, ø, ttem E, Lamark T, Johansen T, NIMA-related kinase 9-mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1. J Biol Chem, 295(5):1240-1260(2020) [pubmed] |
Keyword List
submitter keyword: Microtubule-associated LC3B protein, Autophagy, In vitro phosphorylation |
Contact List
Terje Johansen |
contact affiliation | Molecular Cancer Research Group, Department of Medical Biology, University of Tromsø – The Arctic University of Norway, 9037 Tromsø, Norway |
contact email | terje.johansen@uit.no |
lab head | |
Jack-Ansgar Bruun |
contact affiliation | University of Tromsø |
contact email | jack-ansgar.bruun@uit.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016681
- Label: PRIDE project
- Name: NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1