PXD016681

PXD016681 is an original dataset announced via ProteomeXchange.

Title	NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1
Description	We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or FLAG-tagged kinases immunopurified from transiently transfected HEK293 cells
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:59:23.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack-Ansgar Bruun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-10 07:25:27	ID requested
1	2020-01-23 00:17:29	announced
⏵ 2	2024-10-22 04:59:25	announced	2024-10-22: Updated project metadata.

10.1074/jbc.ra119.010068;

Shrestha BK, Skytte Rasmussen M, Abudu YP, Bruun JA, Larsen KB, Alemu EA, Sj, ø, ttem E, Lamark T, Johansen T, NIMA-related kinase 9-mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1. J Biol Chem, 295(5):1240-1260(2020) [pubmed]

submitter keyword: Microtubule-associated LC3B protein, Autophagy, In vitro phosphorylation

Terje Johansen
contact affiliation	Molecular Cancer Research Group, Department of Medical Biology, University of Tromsø – The Arctic University of Norway, 9037 Tromsø, Norway
contact email	terje.johansen@uit.no
lab head
Jack-Ansgar Bruun
contact affiliation	University of Tromsø
contact email	jack-ansgar.bruun@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016681

PRIDE project URI

• PRIDE
  1. PXD016681
     1. Label: PRIDE project
     2. Name: NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1