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PXD016681

PXD016681 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1
DescriptionWe found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or FLAG-tagged kinases immunopurified from transiently transfected HEK293 cells
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:59:23.163.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJack-Ansgar Bruun
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-10 07:25:27ID requested
12020-01-23 00:17:29announced
22024-10-22 04:59:25announced2024-10-22: Updated project metadata.
Publication List
10.1074/jbc.ra119.010068;
Shrestha BK, Skytte Rasmussen M, Abudu YP, Bruun JA, Larsen KB, Alemu EA, Sj, ø, ttem E, Lamark T, Johansen T, NIMA-related kinase 9-mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1. J Biol Chem, 295(5):1240-1260(2020) [pubmed]
Keyword List
submitter keyword: Microtubule-associated LC3B protein, Autophagy, In vitro phosphorylation
Contact List
Terje Johansen
contact affiliationMolecular Cancer Research Group, Department of Medical Biology, University of Tromsø – The Arctic University of Norway, 9037 Tromsø, Norway
contact emailterje.johansen@uit.no
lab head
Jack-Ansgar Bruun
contact affiliationUniversity of Tromsø
contact emailjack-ansgar.bruun@uit.no
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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