PXD016676

PXD016676 is an original dataset announced via ProteomeXchange.

Title	Thermosensation is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling
Description	Organismal physiology and survival are influenced by environmental conditions and linked to protein quality control. Proteome integrity is achieved by maintaining an intricate balance between protein folding and degradation. In Caenorhabditis elegans, acute heat stress determines cell non-autonomous regulation of chaperone levels. However, how the perception of environmental changes, including physiological temperature, affects protein degradation remains largely unexplored. Here, we show that loss-of-function of dyf-1 in Caenorhabditis elegans associated with dysfunctional sensory neurons leads to defects in both temperature perception and thermal adaptation of the ubiquitin/proteasome system centered on thermosensory AFD neurons. Impaired perception of moderate temperature changes worsens ubiquitin-dependent proteolysis in intestinal cells. Brain-gut communication regulating protein turnover is mediated by upregulation of the insulin-like peptide INS-5 and inhibition of the calcineurin-regulated forkhead-box transcription factor DAF-16/FOXO. Our data indicate that perception of ambient temperature and its neuronal integration is critical for the control of proteome integrity in complex organisms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:40:32.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandra Segref
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-10 03:49:44	ID requested
1	2022-08-12 14:03:45	announced
⏵ 2	2023-11-14 07:40:35	announced	2023-11-14: Updated project metadata.

Segref A, Vakkayil KL, Padvitski T, Li Q, Kroef V, Lormann J, K, ö, rner L, Finger F, Hoppe T, Thermosensation in Caenorhabditis elegans is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling. Nat Commun, 13(1):5874(2022) [pubmed]

curator keyword: Biological

submitter keyword: calcineurin signaling, Ciliated Neurons,Proteostasis, ageing, Insulin signaling, Ubiquitin, aggregation

Thorsten Hoppe
contact affiliation	Institute for Genetics and CECAD Research Center, University of Cologne, Joseph-Stelzmann Str. 26, 50931 Cologne, Germany
contact email	thorsten.hoppe@uni-koeln.de
lab head
Alexandra Segref
contact affiliation	University of Cologne
contact email	asegref@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD016676

PRIDE project URI

• PRIDE
  1. PXD016676
     1. Label: PRIDE project
     2. Name: Thermosensation is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling