PXD016661 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FAM134B oligomerization drives ER membrane scission for ER-phagy |
Description | ER degradation by selective autophagy (ER-phagy) is crucial for ER homeostasis. However, it remains unclear how ER scission is regulated for subsequent autophagosomal sequestration and lysosomal degradation. Here, we show that oligomer formation of FAM134B (also referred to as reticulophagy regulator 1 or RETREG1) through its Reticulon domain was required for membrane fragmentation in vitro and ER-phagy in vivo. Under ER stress conditions, activated CAMK2B phosphorylated the Reticulon domain of FAM134B, which enhanced FAM134B oligomerization and activity in membrane fragmentation to accommodate high demand of ER-phagy. Unexpectedly, FAM134BG216R, a variant derived from a type II HSAN patient, exhibited gain-of-function defects, such as hyperactive self-association and membrane scission, which resulted in excessive ER-phagy and sensory neuron death. Therefore, this study revealed a mechanism of ER membrane fragmentation in ER-phagy, along with a signaling pathway in regulating ER turnover, and suggested a potential implication of excessive selective autophagy in human diseases. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:59:57.221.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jingzi Zhang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos; TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-09 05:30:43 | ID requested | |
1 | 2020-01-09 10:55:17 | announced | |
2 | 2020-01-20 00:42:20 | announced | 2020-01-20: Updated publication reference for PubMed record(s): 31930741. |
⏵ 3 | 2024-10-22 04:59:58 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.15252/embj.2019102608; |
Jiang X, Wang X, Ding X, Du M, Li B, Weng X, Zhang J, Li L, Tian R, Zhu Q, Chen S, Wang L, Liu W, Fang L, Neculai D, Sun Q, FAM134B oligomerization drives endoplasmic reticulum membrane scission for ER-phagy. EMBO J, 39(5):e102608(2020) [pubmed] |
Keyword List
submitter keyword: ER-phagy |
FAM134B oligomerization |
membrane fragmentation |
ER stress |
CAMK2B |
Contact List
Qiming Sun |
contact affiliation | Department of Biochemistry, and Department of Cardiology of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China. |
contact email | qmsun@zju.edu.cn |
lab head | |
Jingzi Zhang |
contact affiliation | Nanjing University |
contact email | zhangjz@nicemice.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016661
- Label: PRIDE project
- Name: FAM134B oligomerization drives ER membrane scission for ER-phagy