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PXD016661

PXD016661 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFAM134B oligomerization drives ER membrane scission for ER-phagy
DescriptionER degradation by selective autophagy (ER-phagy) is crucial for ER homeostasis. However, it remains unclear how ER scission is regulated for subsequent autophagosomal sequestration and lysosomal degradation. Here, we show that oligomer formation of FAM134B (also referred to as reticulophagy regulator 1 or RETREG1) through its Reticulon domain was required for membrane fragmentation in vitro and ER-phagy in vivo. Under ER stress conditions, activated CAMK2B phosphorylated the Reticulon domain of FAM134B, which enhanced FAM134B oligomerization and activity in membrane fragmentation to accommodate high demand of ER-phagy. Unexpectedly, FAM134BG216R, a variant derived from a type II HSAN patient, exhibited gain-of-function defects, such as hyperactive self-association and membrane scission, which resulted in excessive ER-phagy and sensory neuron death. Therefore, this study revealed a mechanism of ER membrane fragmentation in ER-phagy, along with a signaling pathway in regulating ER turnover, and suggested a potential implication of excessive selective autophagy in human diseases.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:59:57.221.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJingzi Zhang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos; TripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-09 05:30:43ID requested
12020-01-09 10:55:17announced
22020-01-20 00:42:20announced2020-01-20: Updated publication reference for PubMed record(s): 31930741.
32024-10-22 04:59:58announced2024-10-22: Updated project metadata.
Publication List
10.15252/embj.2019102608;
Jiang X, Wang X, Ding X, Du M, Li B, Weng X, Zhang J, Li L, Tian R, Zhu Q, Chen S, Wang L, Liu W, Fang L, Neculai D, Sun Q, FAM134B oligomerization drives endoplasmic reticulum membrane scission for ER-phagy. EMBO J, 39(5):e102608(2020) [pubmed]
Keyword List
submitter keyword: ER-phagy
FAM134B oligomerization
membrane fragmentation
ER stress
CAMK2B
Contact List
Qiming Sun
contact affiliationDepartment of Biochemistry, and Department of Cardiology of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.
contact emailqmsun@zju.edu.cn
lab head
Jingzi Zhang
contact affiliationNanjing University
contact emailzhangjz@nicemice.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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