PXD016659

PXD016659 is an original dataset announced via ProteomeXchange.

Title	Light-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria
Description	Covalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to globally monitor their engagement by covalent inhibitors. Here, we present the use of light-activatable 2,5-disubstituted tetrazoles that allow quantifying close to 9000 aspartates and glutamates in the bacterial proteome with excellent selectivity. Using these probes, we competitively map the binding sites of two isoxazolium salts and introduce hydrazonyl chlorides as a new class of carboxylic acid-directed covalent protein ligands. As the probes are unreactive prior to activation, they allow global profiling even in living Gram-positive and Gram-negative bacteria. Taken together, this method to monitor aspartates and glutamates proteome-wide will lay the foundation to efficiently develop covalent inhibitors targeting these amino acids.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:02.260.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan M. Hacker
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-09 02:14:39	ID requested
1	2020-04-13 23:30:23	announced
2	2021-01-06 07:15:39	announced	2021-01-06: Updated publication reference for PubMed record(s): 32342004.
⏵ 3	2024-10-22 05:02:10	announced	2024-10-22: Updated project metadata.

Bach K, Beerkens BLH, Zanon PRA, Hacker SM, Light-Activatable, 2,5-Disubstituted Tetrazoles for the Proteome-wide Profiling of Aspartates and Glutamates in Living Bacteria. ACS Cent Sci, 6(4):546-554(2020) [pubmed]

10.1021/acscentsci.9b01268;

curator keyword: Biological

submitter keyword: Covalent inhibitors,Antibiotics, Chemoproteomics, isoDTB-ABPP

Stephan M. Hacker
contact affiliation	Technical University Munich, Department of Chemistry, Lichtenbergstrasse 4, 85748 Garching bei Muenchen, Germany
contact email	stephan.m.hacker@tum.de
lab head
Stephan M. Hacker
contact affiliation	Technical University of Munich
contact email	stephan.m.hacker@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016659

PRIDE project URI

• PRIDE
  1. PXD016659
     1. Label: PRIDE project
     2. Name: Light-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria