PXD016659 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Light-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria |
Description | Covalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to globally monitor their engagement by covalent inhibitors. Here, we present the use of light-activatable 2,5-disubstituted tetrazoles that allow quantifying close to 9000 aspartates and glutamates in the bacterial proteome with excellent selectivity. Using these probes, we competitively map the binding sites of two isoxazolium salts and introduce hydrazonyl chlorides as a new class of carboxylic acid-directed covalent protein ligands. As the probes are unreactive prior to activation, they allow global profiling even in living Gram-positive and Gram-negative bacteria. Taken together, this method to monitor aspartates and glutamates proteome-wide will lay the foundation to efficiently develop covalent inhibitors targeting these amino acids. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:02:02.260.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephan M. Hacker |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-09 02:14:39 | ID requested | |
1 | 2020-04-13 23:30:23 | announced | |
2 | 2021-01-06 07:15:39 | announced | 2021-01-06: Updated publication reference for PubMed record(s): 32342004. |
⏵ 3 | 2024-10-22 05:02:10 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
curator keyword: Biological |
submitter keyword: Covalent inhibitors,Antibiotics, Chemoproteomics, isoDTB-ABPP |
Contact List
Stephan M. Hacker |
contact affiliation | Technical University Munich, Department of Chemistry, Lichtenbergstrasse 4, 85748 Garching bei Muenchen, Germany |
contact email | stephan.m.hacker@tum.de |
lab head | |
Stephan M. Hacker |
contact affiliation | Technical University of Munich |
contact email | stephan.m.hacker@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016659
- Label: PRIDE project
- Name: Light-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria