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PXD016659

PXD016659 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLight-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria
DescriptionCovalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to globally monitor their engagement by covalent inhibitors. Here, we present the use of light-activatable 2,5-disubstituted tetrazoles that allow quantifying close to 9000 aspartates and glutamates in the bacterial proteome with excellent selectivity. Using these probes, we competitively map the binding sites of two isoxazolium salts and introduce hydrazonyl chlorides as a new class of carboxylic acid-directed covalent protein ligands. As the probes are unreactive prior to activation, they allow global profiling even in living Gram-positive and Gram-negative bacteria. Taken together, this method to monitor aspartates and glutamates proteome-wide will lay the foundation to efficiently develop covalent inhibitors targeting these amino acids.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:02:02.260.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephan M. Hacker
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-09 02:14:39ID requested
12020-04-13 23:30:23announced
22021-01-06 07:15:39announced2021-01-06: Updated publication reference for PubMed record(s): 32342004.
32024-10-22 05:02:10announced2024-10-22: Updated project metadata.
Publication List
10.1021/acscentsci.9b01268;
Keyword List
curator keyword: Biological
submitter keyword: Covalent inhibitors,Antibiotics, Chemoproteomics, isoDTB-ABPP
Contact List
Stephan M. Hacker
contact affiliationTechnical University Munich, Department of Chemistry, Lichtenbergstrasse 4, 85748 Garching bei Muenchen, Germany
contact emailstephan.m.hacker@tum.de
lab head
Stephan M. Hacker
contact affiliationTechnical University of Munich
contact emailstephan.m.hacker@tum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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