PXD016643

PXD016643 is an original dataset announced via ProteomeXchange.

Title	Reversible phosphorylation of Rpn1 regulates 26S proteasome assembly and function
Description	The fundamental importance of the 26S proteasome in health and disease suggests that its function must be finely controlled, and yet our knowledge about proteasome regulation remains limited. Posttranslational modifications, especially phosphorylation, of proteasome subunits have been shown to impact proteasome function through different mechanisms, although the vast majority of proteasome phosphorylation events have not been studied. Here, we have characterized 1 of the most frequently detected proteasome phosphosites, namely Ser361 of Rpn1, a base subunit of the 19S regulatory particle. Using a variety of approaches including CRISPR/Cas9-mediated gene editing and quantitative mass spectrometry, we found that loss of Rpn1-S361 phosphorylation reduces proteasome activity, impairs cell proliferation, and causes oxidative stress as well as mitochondrial dysfunction. A screen of the human kinome identified several kinases including PIM1/2/3 that catalyze S361 phosphorylation, while its level is reversibly controlled by the proteasome-resident phosphatase, UBLCP1. Mechanistically, Rpn1-S361 phosphorylation is required for proper assembly of the 26S proteasome, and we have utilized a genetic code expansion system to directly demonstrate that S361-phosphorylated Rpn1 more readily forms a precursor complex with Rpt2, 1 of the first steps of 19S base assembly. These findings have revealed a prevalent and biologically important mechanism governing proteasome formation and function.
HostingRepository	iProX
AnnounceDate	2019-12-08
AnnouncementXML	Submission_2020-09-03_20:59:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weidi Xiao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-08 05:08:39	ID requested
1	2019-12-08 05:16:47	announced
⏵ 2	2020-09-03 20:59:23	announced	2020-09-04: Update publication information

Liu X, Xiao W, Zhang Y, Wiley SE, Zuo T, Zheng Y, Chen N, Chen L, Wang X, Zheng Y, Huang L, Lin S, Murphy AN, Dixon JE, Xu P, Guo X, Reversible phosphorylation of Rpn1 regulates 26S proteasome assembly and function. Proc Natl Acad Sci U S A, 117(1):328-336(2020) [pubmed]

submitter keyword: PIM, UBLCP1, genetic code expansion, phosphorylation, proteasome

Ping Xu
contact affiliation	Beijing Proteome Research Center
contact email	xuping@mail.ncpsb.org
lab head
Weidi Xiao
contact affiliation	Beijing Proteome Research Center
contact email	weidixiao@126.com
dataset submitter

iProX dataset URI