PXD016640

PXD016640 is an original dataset announced via ProteomeXchange.

Title	Robust ligand-protein interaction inference reveals off-targets of epigenetic drugs
Description	Detecting target engagement is a major challenge in drug discovery. To this end, thermal proteome profiling (TPP) offers unbiased assessment of system-wide ligand-protein interactions. However, its most sensitive assay format lacks statistical methods with false discovery rate-control. Here, we present FILIP, a functional data analysis approach and showcase its performance on several TPP-datasets probing epigenetic drugs. This leads us to identify drug off-targets which we validate in vitro.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:14:26.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nils Kurzawa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-06 06:51:51	ID requested
1	2020-10-28 23:42:00	announced
2	2020-12-04 01:06:58	announced	2020-12-04: Updated publication reference for PubMed record(s): 33188197.
⏵ 3	2024-10-22 05:14:27	announced	2024-10-22: Updated project metadata.

Kurzawa N, Becher I, Sridharan S, Franken H, Mateus A, Anders S, Bantscheff M, Huber W, Savitski MM, A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles. Nat Commun, 11(1):5783(2020) [pubmed]

10.1038/s41467-020-19529-8;

curator keyword: Biological, Biomedical

submitter keyword: TPP, epigenetic drugs

Marcus Bantscheff
contact affiliation	Cellzome, a GSK company, Heidelberg, Germany
contact email	marcus.x.bantscheff@gsk.com
lab head
Nils Kurzawa
contact affiliation	EMBL Heidelberg
contact email	nils.kurzawa@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD016640

PRIDE project URI

• PRIDE
  1. PXD016640
     1. Label: PRIDE project
     2. Name: Robust ligand-protein interaction inference reveals off-targets of epigenetic drugs