PXD016639

PXD016639 is an original dataset announced via ProteomeXchange.

Title	Integrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints
Description	Integrative structure modelling was applied to understand how auto-phosphorylation of the ABCDE cluster in DNA-PKcs can occur. We used crosslinking restraints to assign sequence to structurally unassigned helices in DNA-PKcs s in the largely disordered region containing the ABCDE cluster. Our final model positions the ABCDE cluster at the end of the second helices, suggesting that phosphorylation occurs through trans-auto-phosphorylation, or through a large conformational change in the disordered region for cis-auto-phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2019-12-10
AnnouncementXML	Submission_2019-12-09_23:59:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Morgan Hepburn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-06 06:49:44	ID requested
⏵ 1	2019-12-09 23:59:27	announced

Saltzberg DJ, Hepburn M, Pilla KB, Schriemer DC, Lees-Miller SP, Blundell TL, Sali A, SSEThread: Integrative threading of the DNA-PKcs sequence based on data from chemical cross-linking and hydrogen deuterium exchange. Prog Biophys Mol Biol, 147():92-102(2019) [pubmed]

submitter keyword: Integrative Modelling, Crosslinking-MS, DNA-PKcs

David C. Schriemer
contact affiliation	Biochemistry & Molecular Biology Cumming School of Medicine University of Calgary, Canada
contact email	dschriem@ucalgary.ca
lab head
Morgan Hepburn
contact affiliation	University of Calgary
contact email	mhoeppne@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016639

PRIDE project URI

• PRIDE
  1. PXD016639
     1. Label: PRIDE project
     2. Name: Integrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints