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PXD016639

PXD016639 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints
DescriptionIntegrative structure modelling was applied to understand how auto-phosphorylation of the ABCDE cluster in DNA-PKcs can occur. We used crosslinking restraints to assign sequence to structurally unassigned helices in DNA-PKcs s in the largely disordered region containing the ABCDE cluster. Our final model positions the ABCDE cluster at the end of the second helices, suggesting that phosphorylation occurs through trans-auto-phosphorylation, or through a large conformational change in the disordered region for cis-auto-phosphorylation.
HostingRepositoryPRIDE
AnnounceDate2019-12-10
AnnouncementXMLSubmission_2019-12-09_23:59:26.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMorgan Hepburn
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-06 06:49:44ID requested
12019-12-09 23:59:27announced
Publication List
Saltzberg DJ, Hepburn M, Pilla KB, Schriemer DC, Lees-Miller SP, Blundell TL, Sali A, SSEThread: Integrative threading of the DNA-PKcs sequence based on data from chemical cross-linking and hydrogen deuterium exchange. Prog Biophys Mol Biol, 147():92-102(2019) [pubmed]
Keyword List
submitter keyword: Integrative Modelling, Crosslinking-MS, DNA-PKcs
Contact List
David C. Schriemer
contact affiliationBiochemistry & Molecular Biology Cumming School of Medicine University of Calgary, Canada
contact emaildschriem@ucalgary.ca
lab head
Morgan Hepburn
contact affiliationUniversity of Calgary
contact emailmhoeppne@ucalgary.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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