PXD016639 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints |
Description | Integrative structure modelling was applied to understand how auto-phosphorylation of the ABCDE cluster in DNA-PKcs can occur. We used crosslinking restraints to assign sequence to structurally unassigned helices in DNA-PKcs s in the largely disordered region containing the ABCDE cluster. Our final model positions the ABCDE cluster at the end of the second helices, suggesting that phosphorylation occurs through trans-auto-phosphorylation, or through a large conformational change in the disordered region for cis-auto-phosphorylation. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-10 |
AnnouncementXML | Submission_2019-12-09_23:59:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Morgan Hepburn |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-06 06:49:44 | ID requested | |
⏵ 1 | 2019-12-09 23:59:27 | announced | |
Publication List
Saltzberg DJ, Hepburn M, Pilla KB, Schriemer DC, Lees-Miller SP, Blundell TL, Sali A, SSEThread: Integrative threading of the DNA-PKcs sequence based on data from chemical cross-linking and hydrogen deuterium exchange. Prog Biophys Mol Biol, 147():92-102(2019) [pubmed] |
Keyword List
submitter keyword: Integrative Modelling, Crosslinking-MS, DNA-PKcs |
Contact List
David C. Schriemer |
contact affiliation | Biochemistry & Molecular Biology Cumming School of Medicine University of Calgary, Canada |
contact email | dschriem@ucalgary.ca |
lab head | |
Morgan Hepburn |
contact affiliation | University of Calgary |
contact email | mhoeppne@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016639 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016639
- Label: PRIDE project
- Name: Integrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints