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PXD016632

PXD016632 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive proteome of the adult neural stem cell niche identifies key regulators of adult neurogenesis
DescriptionNeural stem cells (NSCs) generating new neurons are restricted to few niches in the adult mammalian brain, while the remainder rather promotes gliogenesis. Here we take advantage of the spatial separation of an NSC niche (the subependymal zone) and the region to where the newly generated neurons migrate and integrate (the olfactory bulb). Using state-of-the-art mass spectrometry we present a comprehensive proteomic characterization of these niches compared to a region of the normal brain parenchyma (cerebral cortex) that is not contusive to neurogenesis and new neuron integration. We find unique compositions of regulatory ECM components in the neurogenic niche, with quiescent NSCs as a main source of their local ECM, including the multi-functional enzyme transglutaminase 2 that we identify as crucial for neurogenesis. Atomic force microscopy further corroborates indications from the proteome that neurogenic niches are significantly stiffer than the non-neurogenic parenchyma, highlighting the unique properties of these special niches.
HostingRepositoryPRIDE
AnnounceDate2020-01-27
AnnouncementXMLSubmission_2020-02-16_23:08:35.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJacob Kjell
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-06 05:22:01ID requested
12020-01-27 06:55:45announced
22020-02-16 23:08:37announced2020-02-17: Updated publication reference for PubMed record(s): 32032526.
Publication List
Kjell J, Fischer-Sternjak J, Thompson AJ, Friess C, Sticco MJ, Salinas F, Cox J, Martinelli DC, Ninkovic J, Franze K, Schiller HB, G, ö, tz M, Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. Cell Stem Cell, 26(2):277-293.e8(2020) [pubmed]
Keyword List
submitter keyword: Stem cells, Neurogenesis, Extracellular matrix, Brain
Contact List
Magdalena Götz
contact affiliationPysiological Genomics, LMU Muenchen, Germany
contact emailmagdalena.goetz@helmholtz-muenchen.de
lab head
Jacob Kjell
contact affiliationKarolinska Institutet
contact emailjacob.kjell@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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