PXD016619 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fam20C interactome in the secretory pathway under ER stress |
Description | Accumulated unfolded proteins in the endoplasmic reticulum (ER) will trigger the unfolded protein response (UPR) to increase protein-folding capacity. The ER proteostasis and UPR signaling should be precisely and timely regulated. In the project, by unbiased proteomics analysis, we identify that the serine 357 of protein disulfide isomerase (PDI) is rapidly phosphorylated by the secretory pathway kinase Fam20C under ER stress. Remarkably, phosphorylated Ser357 induces an open conformation of PDI and turns it from a ‘foldase’ to a ‘holdase’, which is critical for preventing protein misfolding in the ER. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:50:45.956.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jianchao Zhang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-05 06:47:22 | ID requested | |
1 | 2020-03-02 06:13:35 | announced | |
2 | 2020-03-24 00:29:57 | announced | 2020-03-24: Updated publication reference for PubMed record(s): 32149426. |
3 | 2020-04-01 05:10:28 | announced | 2020-03-24: Updated publication reference for PubMed record(s): 32149426.
2020-04-01: Updated publication reference for PubMed record(s): 32149426. |
⏵ 4 | 2023-11-14 08:50:46 | announced | 2023-11-14: Updated project metadata. |
Publication List
Yu J, Li T, Liu Y, Wang X, Zhang J, Wang X, Shi G, Lou J, Wang L, Wang CC, Wang L, Phosphorylation switches protein disulfide isomerase activity to maintain proteostasis and attenuate ER stress. EMBO J, 39(10):e103841(2020) [pubmed] |
Keyword List
submitter keyword: Fam20C,PDI, ER stress, Phosphorylation |
Contact List
Lei Wang |
contact affiliation | National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China |
contact email | wanglei@ibp.ac.cn |
lab head | |
Jianchao Zhang |
contact affiliation | Institue of Biophysics, Chinese Academy of Sciences |
contact email | zhangjianchao@moon.ibp.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016619
- Label: PRIDE project
- Name: Fam20C interactome in the secretory pathway under ER stress