PXD016619

PXD016619 is an original dataset announced via ProteomeXchange.

Title	Fam20C interactome in the secretory pathway under ER stress
Description	Accumulated unfolded proteins in the endoplasmic reticulum (ER) will trigger the unfolded protein response (UPR) to increase protein-folding capacity. The ER proteostasis and UPR signaling should be precisely and timely regulated. In the project, by unbiased proteomics analysis, we identify that the serine 357 of protein disulfide isomerase (PDI) is rapidly phosphorylated by the secretory pathway kinase Fam20C under ER stress. Remarkably, phosphorylated Ser357 induces an open conformation of PDI and turns it from a ‘foldase’ to a ‘holdase’, which is critical for preventing protein misfolding in the ER.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:45.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianchao Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-05 06:47:22	ID requested
1	2020-03-02 06:13:35	announced
2	2020-03-24 00:29:57	announced	2020-03-24: Updated publication reference for PubMed record(s): 32149426.
3	2020-04-01 05:10:28	announced	2020-03-24: Updated publication reference for PubMed record(s): 32149426. 2020-04-01: Updated publication reference for PubMed record(s): 32149426.
⏵ 4	2023-11-14 08:50:46	announced	2023-11-14: Updated project metadata.

Yu J, Li T, Liu Y, Wang X, Zhang J, Wang X, Shi G, Lou J, Wang L, Wang CC, Wang L, Phosphorylation switches protein disulfide isomerase activity to maintain proteostasis and attenuate ER stress. EMBO J, 39(10):e103841(2020) [pubmed]

submitter keyword: Fam20C,PDI, ER stress, Phosphorylation

Lei Wang
contact affiliation	National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	wanglei@ibp.ac.cn
lab head
Jianchao Zhang
contact affiliation	Institue of Biophysics, Chinese Academy of Sciences
contact email	zhangjianchao@moon.ibp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016619

PRIDE project URI

• PRIDE
  1. PXD016619
     1. Label: PRIDE project
     2. Name: Fam20C interactome in the secretory pathway under ER stress