PXD016616

PXD016616 is an original dataset announced via ProteomeXchange.

Title	Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective
Description	We performed a combination of metabolic engineering (deletion of ldh and poxB and overexpression of mmc) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in Propionibacterium acidipropionici. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from P. acidipropionici-Δldh-ΔpoxB+mmc by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of PA titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the 5.5-fold upregulation of Dps, 2 to 4-fold upregulation of ABC-type glycine betaine transporter and 3-fold upregulation of SOD indicated that these genes were likely in key regulons for the adaptation to abiotic stresses. An approximately 2.5-fold upregulation of mmc was also found. The results showed the multidirectional variation tendency of P. acidipropionici under cross stress and provide in-depth insights into the mechanism of tolerance and high production of PA.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-04-27_00:45:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tingting Liu
SpeciesList	scientific name: Acidipropionibacterium acidipropionici; NCBI TaxID: 1748;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-05 06:46:58	ID requested
⏵ 1	2020-04-27 00:45:08	announced

Dataset with its publication pending

submitter keyword: Propionibacterium acidipropionici, Propionic acid, Metabolic and evolutionary engineering, Cross stress, Omics analysis

Tingting Liu
contact affiliation	College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University
contact email	709301295@qq.com
lab head
Tingting Liu
contact affiliation	Nanjing Tech University
contact email	709301295@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016616

PRIDE project URI

• PRIDE
  1. PXD016616
     1. Label: PRIDE project
     2. Name: Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective