PXD016615

PXD016615 is an original dataset announced via ProteomeXchange.

Title	The Arabidopsis Mediator complex subunit 8 regulates oxidative stress responses
Description	Hydrogen peroxide (H2O2) signals regulate plant growth and defense by orchestrating genome-wide transcriptional re-programming, however, the specific mechanisms that regulate H2O2-dependent gene expression are poorly understood. Here we report the identification of the Mediator complex subunit MED8 as regulator of H2O2 responses, through a genetic screen of EAL4 promoter. Interestingly, the introduction of med8 mutation in the catalase-deficient background was associated with enhanced activation of salicylic acid biosynthesis and signaling pathways. Surprisingly, however, med8 seedlings were more tolerant to oxidative stress generated by methyl viologen. This tolerance phenotype is associated with the hyperactivation of defense and hormone signaling pathways in particular salicylic acid and jasmonic acid-related pathways. In addition, analysis of MED8 interactomes revealed interaction with novel pathways expanding Mediator complex function to processes beyond transcription, including miRNA biogenesis and and mRNA processing. We establish MED8 as a new component within the regulation of oxidative stress responses and demonstrate that MED8 act as negative regulator of H2O2 -driven activation of defense gene expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:26.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Willems
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-05 06:46:58	ID requested
1	2021-09-17 04:11:00	announced
⏵ 2	2024-10-22 05:29:27	announced	2024-10-22: Updated project metadata.

10.1093/plcell/koab079;

He H, Denecker J, Van Der Kelen K, Willems P, Pottie R, Phua SY, Hannah MA, Vertommen D, Van Breusegem F, Mhamdi A, The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses. Plant Cell, 33(6):2032-2057(2021) [pubmed]

submitter keyword: Arabidopsis,Mediator complex, catalase, oxidative stress, MED8, hydrogen peroxide

Frank Van Breusegem
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Oxidative Stress Signalling lab, Ghent University
contact email	frbre@psb.ugent.be
lab head
Patrick Willems
contact affiliation	PSB-VIB
contact email	pawil@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD016615

PRIDE project URI

• PRIDE
  1. PXD016615
     1. Label: PRIDE project
     2. Name: The Arabidopsis Mediator complex subunit 8 regulates oxidative stress responses