PXD016614

PXD016614 is an original dataset announced via ProteomeXchange.

Title	Integrative proteome and acetylome analyses of murine responses to Cryptococcus neoformans infection
Description	Cryptococcus neoformans, an environmental opportunistic human fungal pathogen, is a causative agent for acute pulmonary infection and meningoencephalitis. Understanding the host’s response to C. neoformans infection is critical for developing effective treatment. Even though some have elucidated the host response at the transcriptome level, little is known about how it modulates its defense machinery through the proteome mechanism or how protein posttranslational modification responds to the infection. In this work, we employed a Cryptococcus murine infection model and mass spectrometry to systematically determine the proteome and acetylome statuses of two primary organs (lung and brain) in the early stage of infection. To extensively analyze the host response, we compared and integrated the proteome data to the transcriptome results. We demonstrated a significant overlap between two data sets. Critical genes, including genes involved in phagosome, lysosome, or osteoblast differentiation and platelet activation are significantly altered in protein and gene expression during infection. In the acetylome analysis, we demonstrated that lung and brain tissues differentially regulate protein acetylation during infection. The three primary groups of proteins altered in acetylation status are histones, proteins involved in glucose and fatty acid metabolism, and proteins from the immune system. These analyses provide an integrative regulation network of the host responding to C. neoformans and shed new light on understanding the host’s regulation mechanism when responding to C. neoformans.
HostingRepository	PRIDE
AnnounceDate	2020-04-21
AnnouncementXML	Submission_2020-07-14_23:34:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hai-Long Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-05 06:46:56	ID requested
1	2020-04-21 01:32:21	announced
⏵ 2	2020-07-14 23:34:33	announced	2020-07-15: Updated publication reference for PubMed record(s): 32362878.

Li H, Li Y, Sun T, Du W, Zhang Z, Li D, Ding C, Infection. Front Microbiol, 11():575(2020) [pubmed]

submitter keyword: cryptococcus neoformans infection, lysine acetylome, mouse brain, mouse lung

Chen Ding
contact affiliation	College of Life and Health Sciences, Northeastern University, Liaoning, Shenyang, China
contact email	dingchen@mail.neu.edu.cn
lab head
Hai-Long Li
contact affiliation	Northeastern University
contact email	lihailong1166@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016614

PRIDE project URI

• PRIDE
  1. PXD016614
     1. Label: PRIDE project
     2. Name: Integrative proteome and acetylome analyses of murine responses to Cryptococcus neoformans infection