PXD016607

PXD016607 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis SME interactome reveals spliceosome components
Description	Alternative splicing (AS) can produce multiple transcripts with different exon-intron structures from a single pre-mRNA. Pre-mRNA splicing is catalyzed by a dynamic macromolecular ribonucleoprotein (RNP) complex termed the spliceosome. The spliceosome consists of several small nuclear ribonucleoproteins (snRNPs) that bind uridine-rich small nuclear RNA (snRNA). In U1, U2, U4 and U5 snRNPs, snRNA interacts with the conserved Smith antigen (Sm) proteins via a bipartite Sm sequence motif. In eukaryotes, seven Sm proteins (B, D1/2/3, E, F and G) form a heptameric ring-shaped complex surrounding the snRNA. Here, we performed a tandem affinity purification using SMEB as a bait in Arabidopsis cell suspension cultures. At least 45 known/hypothesized and potential novel spliceosome components were identified in Arabidopsis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:42:25.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Willems
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monomethylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 08:06:28	ID requested
1	2023-03-03 00:33:30	announced
⏵ 2	2024-10-22 05:42:25	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: pre-mRNA splicing, SME,Arabidopsis, Sm ring, spliceosome, splicing, alternative splicing

Frank Van Breusegem
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Oxidative Stress Signaling Lab, Ghent University
contact email	frbre@psb.ugent.be
lab head
Patrick Willems
contact affiliation	PSB-VIB
contact email	pawil@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD016607

PRIDE project URI

• PRIDE
  1. PXD016607
     1. Label: PRIDE project
     2. Name: Arabidopsis SME interactome reveals spliceosome components