PXD016605 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immediate adaption – Quantitative Proteomics of BCL6 silencing and EGFR TK inhibition |
Description | We aimed to identify the response mechanism to EGFR tyrosine kinase inhibition. A431 cells were transfected with mock siRNA or BCL6 siRNA. Cells were then treated with gefitinib. Harvesting was done at 0h, at 24h and at 48h. Two TMT10plex sets were organized as follows: TMT set1: mock 0h (3x, channels 126, 127N and 127C), mock 24h (3x, 128N, 128C and 129N), mock 48h (3x, 129C, 130N, 130C) and internal pooled standard (131) composed from equal aliquots of all samples (mock siRNA and BCL6 siRNA). TMT set2: siBCL6 0h (3x, channels 126, 127N and 127C), siBCL6 24h (3x, 128N, 128C and 129N), siBCL6 48h (3x, 129C, 130N, 130C) and the same internal pooled standard (131) as for set 1. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:16:25.969.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Branca |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-04 08:06:22 | ID requested | |
1 | 2020-12-09 00:55:53 | announced | |
⏵ 2 | 2024-10-22 05:16:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra120.002036; |
Zhou Tran Y, Minozada R, Cao X, Johansson HJ, Branca RM, Seashore-Ludlow B, Orre LM, Immediate Adaptation Analysis Implicates BCL6 as an EGFR-TKI Combination Therapy Target in NSCLC. Mol Cell Proteomics, 19(6):928-943(2020) [pubmed] |
Keyword List
submitter keyword: Human, BCL6 siRNA, TMT proteomics, LC-MS, time course, EGFR inhibition, gefitinib, A431 cells |
Contact List
Janne Lehtiö |
contact affiliation | Dept. Oncology Pathology, Karolinska Institutet, Scilifelab, Stockholm, Sweden |
contact email | janne.lehtio@ki.se |
lab head | |
Rui Branca |
contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
contact email | rui.mamede-branca@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016605
- Label: PRIDE project
- Name: Immediate adaption – Quantitative Proteomics of BCL6 silencing and EGFR TK inhibition