PXD016602

PXD016602 is an original dataset announced via ProteomeXchange.

Title	Hap2-Ino80 mediated transcription facilitates de novo establishment of CENP-A chromatin
Description	Centromeres are maintained epigenetically by the presence of CENP-A, an evolutionarily-conserved histone H3 variant, which directs kinetochore assembly and hence, centromere function. To identify factors that promote assembly of CENP-A chromatin, we affinity selected solubilised fission yeast CENP-ACnp1 chromatin. All subunits of the Ino80 complex were enriched, including the auxiliary subunit Hap2. In addition to a role in maintenance of CENP-ACnp1 chromatin integrity at endogenous centromeres, Hap2 is required for de novo assembly of CENP-ACnp1 chromatin on naïve centromere DNA and promotes H3 turnover on centromere regions and other loci prone to CENP-ACnp1 deposition. Prior to CENP-ACnp1 chromatin assembly, Hap2 is required for transcription from centromere DNA. These analyses suggest that Hap2-Ino80 destabilises H3 nucleosomes on centromere DNA through transcription-mediated histone H3 turnover, driving the replacement of resident H3 nucleosomes with CENP-ACnp1 nucleosomes. These inherent properties define centromere DNA by directing a program that mediates CENP-ACnp1 assembly on appropriate sequences.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:05:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Puneet Singh
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 08:06:01	ID requested
⏵ 1	2020-05-26 16:05:56	announced

Singh PP, Shukla M, White SA, Lafos M, Tong P, Auchynnikava T, Spanos C, Rappsilber J, Pidoux AL, Allshire RC, Hap2-Ino80-facilitated transcription promotes de novo establishment of CENP-A chromatin. Genes Dev, 34(3-4):226-238(2020) [pubmed]

submitter keyword: Centromeres, nucleosomes, chromatin, CENP-A, histones, label-free proteomics

Robin Allshire
contact affiliation	Wellcome Centre for Cell Biology, University of Edinburgh
contact email	Robin.Allshire@ed.ac.uk
lab head
Puneet Singh
contact affiliation	The University of Edinburgh
contact email	puneetedi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016602

PRIDE project URI

• PRIDE
  1. PXD016602
     1. Label: PRIDE project
     2. Name: Hap2-Ino80 mediated transcription facilitates de novo establishment of CENP-A chromatin