PXD016602 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hap2-Ino80 mediated transcription facilitates de novo establishment of CENP-A chromatin |
Description | Centromeres are maintained epigenetically by the presence of CENP-A, an evolutionarily-conserved histone H3 variant, which directs kinetochore assembly and hence, centromere function. To identify factors that promote assembly of CENP-A chromatin, we affinity selected solubilised fission yeast CENP-ACnp1 chromatin. All subunits of the Ino80 complex were enriched, including the auxiliary subunit Hap2. In addition to a role in maintenance of CENP-ACnp1 chromatin integrity at endogenous centromeres, Hap2 is required for de novo assembly of CENP-ACnp1 chromatin on naïve centromere DNA and promotes H3 turnover on centromere regions and other loci prone to CENP-ACnp1 deposition. Prior to CENP-ACnp1 chromatin assembly, Hap2 is required for transcription from centromere DNA. These analyses suggest that Hap2-Ino80 destabilises H3 nucleosomes on centromere DNA through transcription-mediated histone H3 turnover, driving the replacement of resident H3 nucleosomes with CENP-ACnp1 nucleosomes. These inherent properties define centromere DNA by directing a program that mediates CENP-ACnp1 assembly on appropriate sequences. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:05:50.646.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Puneet Singh |
SpeciesList | scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-04 08:06:01 | ID requested | |
1 | 2020-05-26 16:05:56 | announced | |
⏵ 2 | 2024-10-22 05:05:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1101/gad.332536.119; |
Singh PP, Shukla M, White SA, Lafos M, Tong P, Auchynnikava T, Spanos C, Rappsilber J, Pidoux AL, Allshire RC, Hap2-Ino80-facilitated transcription promotes de novo establishment of CENP-A chromatin. Genes Dev, 34(3-4):226-238(2020) [pubmed] |
Keyword List
submitter keyword: CENP-A, histones, label-free proteomics,Centromeres, nucleosomes, chromatin |
Contact List
Robin Allshire |
contact affiliation | Wellcome Centre for Cell Biology, University of Edinburgh |
contact email | Robin.Allshire@ed.ac.uk |
lab head | |
Puneet Singh |
contact affiliation | The University of Edinburgh |
contact email | puneetedi@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016602
- Label: PRIDE project
- Name: Hap2-Ino80 mediated transcription facilitates de novo establishment of CENP-A chromatin