PXD016600

PXD016600 is an original dataset announced via ProteomeXchange.

Title	Human NEK10 knockout airway ALI Fe-IMAC-MSMS
Description	We identified inactivating mutations in NEK10, a poorly characterized human protein kinase, in a novel human bronchiectasis syndrome. In order to understand effects of loss of its function on the airway phosphoproteome, NEK10 was CRISPR/Cas9 targeted in human airway air-liquid interface (ALI) cultures with 2 independent guides and assayed by iron-enrichment phosphoproteomics.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-02-17_02:26:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raghu Chivukula
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 08:01:33	ID requested
1	2020-01-09 10:20:30	announced
⏵ 2	2020-02-17 02:26:12	announced	2020-02-17: Updated publication reference for PubMed record(s): 31959991.

Chivukula RR, Montoro DT, Leung HM, Yang J, Shamseldin HE, Taylor MS, Dougherty GW, Zariwala MA, Carson J, Daniels MLA, Sears PR, Black KE, Hariri LP, Almogarri I, Frenkel EM, Vinarsky V, Omran H, Knowles MR, Tearney GJ, Alkuraya FS, Sabatini DM, A human ciliopathy reveals essential functions for NEK10 in airway mucociliary clearance. Nat Med, 26(2):244-251(2020) [pubmed]

submitter keyword: Human, airway, NEK10, phosphoproteomics

David M. Sabatini
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA
contact email	sabatini@wi.mit.edu
lab head
Raghu Chivukula
contact affiliation	Whitehead Institute for Biomedical Research
contact email	raghu@wi.mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016600

PRIDE project URI

• PRIDE
  1. PXD016600
     1. Label: PRIDE project
     2. Name: Human NEK10 knockout airway ALI Fe-IMAC-MSMS