PXD016595

PXD016595 is an original dataset announced via ProteomeXchange.

Title	Kinetic Analysis of DNA Dependent Protein Kinase Catalytic Subunit
Description	We investigated the dynamics of protein kinase DNA dependent protein kinase catalytic subunit (DNA-PKcs) through hydrogen deuterium exchange mass spectrometry. Over 1100 peptides could be monitored throughout the kinetics experiment, showing the utility of our nanospray HX-MS configuration for ultra-large proteins such as DNA-PKcs. To match the structurally unassigned helices to sequence from the large disordered regions of DNA-PKcs (a.a 2577-2773), we used integrative modelling. Two sequence assignments were possible. The cluster of models with the helix assignment towards the N-terminal side was favored by reduced kinetics of exchange as measured by HX-MS.
HostingRepository	PRIDE
AnnounceDate	2019-12-10
AnnouncementXML	Submission_2019-12-09_23:44:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Morgan Hepburn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 06:33:37	ID requested
⏵ 1	2019-12-09 23:45:00	announced

Sheff JG, Hepburn M, Yu Y, Lees-Miller SP, Schriemer DC, Nanospray HX-MS configuration for structural interrogation of large protein systems. Analyst, 142(6):904-910(2017) [pubmed]

Saltzberg DJ, Hepburn M, Pilla KB, Schriemer DC, Lees-Miller SP, Blundell TL, Sali A, SSEThread: Integrative threading of the DNA-PKcs sequence based on data from chemical cross-linking and hydrogen deuterium exchange. Prog Biophys Mol Biol, 147():92-102(2019) [pubmed]

submitter keyword: HDX-MS, Nanospray HX-MS, DNA-PKcs, Human,

David C. Schriemer
contact affiliation	Biochemistry & Molecular Biology Cumming School of Medicine University of Calgary, Canada
contact email	dschriem@ucalgary.ca
lab head
Morgan Hepburn
contact affiliation	University of Calgary
contact email	mhoeppne@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016595

PRIDE project URI

• PRIDE
  1. PXD016595
     1. Label: PRIDE project
     2. Name: Kinetic Analysis of DNA Dependent Protein Kinase Catalytic Subunit