PXD016592

PXD016592 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	gender-related secretome of Plasmodium berghei sexual stages
Description	In this study, we applied a proteomic approach to identify proteins differentially released/secreted by female and male gametocytes of the rodent Plasmodium berghei activated to form gametes. We compared secreted molecules of the wild type gametocytes with those of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive dataset of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules.
HostingRepository	MassIVE
AnnounceDate	2024-12-18
AnnouncementXML	Submission_2024-12-18_12:03:40.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FEDERICA FRATINI
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 05:44:55	ID requested
⏵ 1	2024-12-18 12:03:41	announced

Publication List

Grasso F, Mochi S, Fratini F, Olivieri A, Curr, à C, Siden Kiamos I, Deligianni E, Birago C, Picci L, Pizzi E, Pace T, Ponzi M, Sexual Stages. Mol Cell Proteomics, 19(12):1986-1997(2020) [pubmed]
Grasso F, Fratini F, Albanese TG, Mochi S, Ciardo M, Pace T, Ponzi M, Pizzi E, Olivieri A, Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation. Sci Rep, 12(1):9592(2022) [pubmed]

Grasso F, Mochi S, Fratini F, Olivieri A, Curr, à C, Siden Kiamos I, Deligianni E, Birago C, Picci L, Pizzi E, Pace T, Ponzi M, Sexual Stages. Mol Cell Proteomics, 19(12):1986-1997(2020) [pubmed]

Grasso F, Fratini F, Albanese TG, Mochi S, Ciardo M, Pace T, Ponzi M, Pizzi E, Olivieri A, Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation. Sci Rep, 12(1):9592(2022) [pubmed]

Keyword List

submitter keyword: SECRETOME, VESICLES, PLASMODUM BERGHEI, GAMETOCYTES

submitter keyword: SECRETOME, VESICLES, PLASMODUM BERGHEI, GAMETOCYTES

Contact List

FEDERICA FRATINI
contact affiliation	ISS
contact email	federica.fratini@iss.it
lab head
FEDERICA FRATINI
contact affiliation	ISS
contact email	federica.fratini@iss.it
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v02/MSV000084655/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v02/MSV000084655/