PXD016588

PXD016588 is an original dataset announced via ProteomeXchange.

Title	Expression of proteins by a marine Brevibacterium casei #NIOSBA88 in response to Arsenic and Chromium
Description	A marine bacterial isolate, Brevibacterium casei #NIOSBA88 was grown separately in Luria Bertini broth supplemented with 750 mg L-1 Arsenic (III) and 100 mg L-1 Chromium respectively. Similarly, the strain was grown in absence of Arsenic and Chromium, which served as an experimental control. The cells were harvested at different time intervals and proteins were extracted from the cell pellets using in-house protocol (Shah and Damare 2018). The extracted proteins were digested using trypsin following standard protocol. Tryptic digests were injected in LCMS QToF for obtaining spectral library which was used to identify the proteins against a species specific database in NCBI.
HostingRepository	PRIDE
AnnounceDate	2020-10-21
AnnouncementXML	Submission_2020-10-21_03:30:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samir Damare
SpeciesList	scientific name: Brevibacterium casei; NCBI TaxID: 33889;
ModificationList	iodoacetamide derivatized residue
Instrument	6538 Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 04:22:45	ID requested
⏵ 1	2020-10-21 03:30:30	announced

Shah S, Damare S, Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium-a proteomic approach. Braz J Microbiol, 51(4):1885-1895(2020) [pubmed]

submitter keyword: LCMS QToF, Trypsin digestion, Peptide mass fingerprinting

Dr. Samir R Damare
contact affiliation	Biological Oceanography Division, CSIR-National Insititute of Ocenography
contact email	samir@nio.org
lab head
Samir Damare
contact affiliation	CSIR-National Institute of Oceanography
contact email	samir@nio.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016588
     1. Label: PRIDE project
     2. Name: Expression of proteins by a marine Brevibacterium casei #NIOSBA88 in response to Arsenic and Chromium