PXD016580

PXD016580 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation ratio of pT vs pY ERK in MEK phosphorylation reaction determined by mass spectrometry
Description	The purpose of this experiment is to determine the phosphorylation ratio of pT vs pY ERK present in ERK phosphorylation reaction by activated MEK E203K or MEK WildType. Aliquots were collected at 3 minutes 15 seconds, the time at which the mono-phosphorylated ERK is at its maximal. To determine the ratio between pT and pY ERK, synthetic AQUA peptides corresponding to the pY- and the pT-phosphorylated ERK were spiked into the sample. The two peptides have identical mass but they can be umabiguguously distinguished by their elution times and fragmentation spectra.
HostingRepository	PRIDE
AnnounceDate	2020-01-14
AnnouncementXML	Submission_2020-01-13_23:55:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eyan Yeung
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 03:33:13	ID requested
⏵ 1	2020-01-13 23:55:20	announced

Dataset with its publication pending

submitter keyword: Recombinant, Kinase reaction, LC-MSMS, Orbitrap Fusion Lumos

Martin Wühr
contact affiliation	Princeton University, Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics
contact email	wuhr@princeton.edu
lab head
Eyan Yeung
contact affiliation	Princeton University
contact email	eyanyeung@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016580
     1. Label: PRIDE project
     2. Name: Phosphorylation ratio of pT vs pY ERK in MEK phosphorylation reaction determined by mass spectrometry