PXD016565

PXD016565 is an original dataset announced via ProteomeXchange.

Title	Secretome and intracellular proteome of espB deletion mutant of Mycobacterium tuberculosis
Description	Mycobacterium tuberculosis relies on the ESX-1 secretion system for survival, multiplication in and escape from macrophages. In this work we investigate the role of the EspB protein, encoded within the esx-1 gene cluster, in virulence and ESX-1 substrate secretion in M. tuberculosis H37Rv. Genetic, proteomic and immunological data show that, contrary to previous reports, an espB knock-out mutant is only partially attenuated in ex vivo infection models, where EsxA, EsxB and EspC antigen presentation is not affected, and secretes the major virulence factor EsxA. Additionally, we demonstrate that EspB does not require an intact and functional ESX-1 apparatus for being secreted in H37Rv and in M. bovis BCG, as opposed to other strains such as CDC1551 and Erdman, thereby suggesting that other ESX systems may be involved in the process. Overall our findings highlight unexplored differences in the secretion profiles of various mycobacterial strains and underscore the plasticity of ESX-dependent secretion in mycobacteria.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:37.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrej Benjak
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 03:23:32	ID requested
1	2021-09-24 01:35:03	announced
⏵ 2	2024-10-22 05:29:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ESX-1, espB,Mycobacterium tuberculosis

Stewart T. Cole
contact affiliation	Institut Pasteur, Paris, France
contact email	stewart.cole@pasteur.fr
lab head
Andrej Benjak
contact affiliation	EPFL
contact email	abenjak@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016565
     1. Label: PRIDE project
     2. Name: Secretome and intracellular proteome of espB deletion mutant of Mycobacterium tuberculosis