PXD016561

PXD016561 is an original dataset announced via ProteomeXchange.

Title	Characterization of Fasciola heptica extracellular vesicles recovered by gravity flow
Description	Parasite-derived extracellular vesicles (EVs) are now at the forefront of helminth research, with evidence indicating that they exhibit immune modulatory activity, presenting a strong case to look at their potential as vaccine candidates. In the present study, Fasciola hepatica derived EVs (FhEVs) were isolated from adult worms using a gravity flow method. These gravity flow derived FhEVs were characterised by proteomics and lectin array. Comparative analysis using the same methods was carried out using FhEVs recovered by differential centrifugation. The immunomodulatory properties of the gravity flow derived FhEVs was determined using bone marrow derived murine dendritic cells (BMDCs), which highlighted that the FhEVs induce a novel phenotype that secreted low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L and SIGNR1) and intracellular markers (SOCS1 and SOCS3). In conclusion, this study demonstrates that FhEVs modulate immune cells, inducing a unique DC phenotype that supresses IL-2 secretion from T-cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_03:54:30.007.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016561
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Krystyna Cwiklinski
SpeciesList	scientific name: Fasciola hepatica (Liver fluke); NCBI TaxID: 6192;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 02:12:41	ID requested
⏵ 1	2024-10-11 03:54:30	announced

10.1371/journal.pntd.0008626;

10.6019/PXD016561;

Murphy A, Cwiklinski K, Lalor R, O'Connell B, Robinson MW, Gerlach J, Joshi L, Kilcoyne M, Dalton JP, O'Neill SM, Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity. PLoS Negl Trop Dis, 14(9):e0008626(2020) [pubmed]

submitter keyword: Fasciola hepatica

extracellular vesicles

excreted-secreted proteins

gravity flow

John P Dalton
contact affiliation	Department of Zoology, School of Natural Sciences, National University of Ireland, Galway.
contact email	johnpius.dalton@nuigalway.ie
lab head
Krystyna Cwiklinski
contact affiliation	NUI Galway
contact email	krystyna.cwiklinski@nuigalway.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD016561

PRIDE project URI

• PRIDE
  1. PXD016561
     1. Label: PRIDE project
     2. Name: Characterization of Fasciola heptica extracellular vesicles recovered by gravity flow