PXD016552

PXD016552 is an original dataset announced via ProteomeXchange.

Title	Monosomes actively translate synaptic mRNAs in neuronal processes
Description	In order to deal with their huge volume and complex morphology, neurons localize mRNAs and ribosomes near synapses to produce proteins locally. A relative paucity of polyribosomes (considered the active sites of translation) detected in electron micrographs of neuronal processes (axons and dendrites), however, has suggested a rather limited capacity for local protein synthesis. Polysome profiling together with ribosome footprinting of microdissected synaptic regions revealed that a surprisingly high number of dendritic and/or axonal transcripts were predominantly associated with monosomes (single ribosomes). Contrary to prevailing views, the neuronal monosomes were in the process of active protein synthesis (e.g. they exhibited elongation). Most mRNAs showed a similar translational status in the somata and neuropil, but some transcripts exhibited differential ribosome occupancy in the compartments. Strikingly, monosome-preferring transcripts often encoded high-abundance synaptic proteins. These data suggest a significant contribution of monosome translation to the maintenance of the local neuronal proteome. This mode of translation can presumably solve some of restricted space issues (given the large size of polysomes) and also increase the diversity of proteins made from a limited number of ribosomes available in dendrites and axons.
HostingRepository	PRIDE
AnnounceDate	2020-01-27
AnnouncementXML	Submission_2020-02-17_03:22:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-03 04:39:52	ID requested
1	2020-01-27 00:37:23	announced
⏵ 2	2020-02-17 03:22:29	announced	2020-02-17: Updated publication reference for PubMed record(s): 32001627.

Biever A, Glock C, Tushev G, Ciirdaeva E, Dalmay T, Langer JD, Schuman EM, Monosomes actively translate synaptic mRNAs in neuronal processes. Science, 367(6477):(2020) [pubmed]

curator keyword: Biological

submitter keyword: monosomes, mRNA, neuronal processes, protein synthesis

Julian Langer
contact affiliation	MPIs for Brain Research and Biophysics
contact email	julian.langer@brain.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016552

PRIDE project URI

• PRIDE
  1. PXD016552
     1. Label: PRIDE project
     2. Name: Monosomes actively translate synaptic mRNAs in neuronal processes