PXD016551

PXD016551 is an original dataset announced via ProteomeXchange.

Title	Positive effect of Manganese on growth of two strains of Granulicella sp. revealed by proteomic and genomic analyses
Description	Members of Acidobacteria phylum are difficult to isolate and grow. Nevertheless, for some isolated strains, trace elements solution is needed in culture media for Acidobacteria growth but the impact of these metals on their growth and metabolism is not known. Here we evaluated the effect of trace element solution SL-10 in on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to Granulicella sp. and studied the proteomic responses to the metal with the best growth results, complementing with genomic characterization. Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal ions. Variations in tolerance to metal ion concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. The proteomic profiles of both strains indicated that Mn was more important in enhance enzymatic activity than to protein expression regulation. We did not find the most common transcriptional regulation of Mn homeostasis and we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment where these two strains were isolated. Furthermore, strain 5B5 is likely more adapted to survive in an environment with higher concentration of several metal ions when compared to strain WH15.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:56.795.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Granulicella sp.; NCBI TaxID: 1969471;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-03 04:39:20	ID requested
1	2021-02-14 23:20:12	announced
⏵ 2	2024-10-22 05:18:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Metabolism,Acidobacteria, Genome, Granulicella, Proteome, Manganese

Kathrin Riedel
contact affiliation	University of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology
contact email	riedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	University of Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016551
     1. Label: PRIDE project
     2. Name: Positive effect of Manganese on growth of two strains of Granulicella sp. revealed by proteomic and genomic analyses