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PXD016551

PXD016551 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePositive effect of Manganese on growth of two strains of Granulicella sp. revealed by proteomic and genomic analyses
DescriptionMembers of Acidobacteria phylum are difficult to isolate and grow. Nevertheless, for some isolated strains, trace elements solution is needed in culture media for Acidobacteria growth but the impact of these metals on their growth and metabolism is not known. Here we evaluated the effect of trace element solution SL-10 in on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to Granulicella sp. and studied the proteomic responses to the metal with the best growth results, complementing with genomic characterization. Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal ions. Variations in tolerance to metal ion concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. The proteomic profiles of both strains indicated that Mn was more important in enhance enzymatic activity than to protein expression regulation. We did not find the most common transcriptional regulation of Mn homeostasis and we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment where these two strains were isolated. Furthermore, strain 5B5 is likely more adapted to survive in an environment with higher concentration of several metal ions when compared to strain WH15.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:18:56.795.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniela Zuehlke
SpeciesList scientific name: Granulicella sp.; NCBI TaxID: 1969471;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-03 04:39:20ID requested
12021-02-14 23:20:12announced
22024-10-22 05:18:57announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Metabolism,Acidobacteria, Genome, Granulicella, Proteome, Manganese
Contact List
Kathrin Riedel
contact affiliationUniversity of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology
contact emailriedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliationUniversity of Greifswald, Institute of Microbiology
contact emailzuehlke@uni-greifswald.de
dataset submitter
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