PXD016551 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Positive effect of Manganese on growth of two strains of Granulicella sp. revealed by proteomic and genomic analyses |
Description | Members of Acidobacteria phylum are difficult to isolate and grow. Nevertheless, for some isolated strains, trace elements solution is needed in culture media for Acidobacteria growth but the impact of these metals on their growth and metabolism is not known. Here we evaluated the effect of trace element solution SL-10 in on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to Granulicella sp. and studied the proteomic responses to the metal with the best growth results, complementing with genomic characterization. Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal ions. Variations in tolerance to metal ion concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. The proteomic profiles of both strains indicated that Mn was more important in enhance enzymatic activity than to protein expression regulation. We did not find the most common transcriptional regulation of Mn homeostasis and we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment where these two strains were isolated. Furthermore, strain 5B5 is likely more adapted to survive in an environment with higher concentration of several metal ions when compared to strain WH15. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:18:56.795.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Granulicella sp.; NCBI TaxID: 1969471; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-03 04:39:20 | ID requested | |
1 | 2021-02-14 23:20:12 | announced | |
⏵ 2 | 2024-10-22 05:18:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Metabolism,Acidobacteria, Genome, Granulicella, Proteome, Manganese |
Contact List
Kathrin Riedel |
contact affiliation | University of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology |
contact email | riedela@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | University of Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD016551 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016551
- Label: PRIDE project
- Name: Positive effect of Manganese on growth of two strains of Granulicella sp. revealed by proteomic and genomic analyses