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PXD016519 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleYeast cell cycle proteome and phosphoproteome (LC-MSMS)
DescriptionMulti-omic absolute quantitative analysis of the yeast (Saccharomyces cerevisiae) mitotic cell cycle. Transcriptomics (RNA-Seq), proteomics (SILAC/ iBAQ), phosphoproteomics (SILAC/ iBAQ, enrichment with TiO2), and untargeted metabolomics (Metabolon, Inc.) were performed, all in biological triplicate. Three sub-projects from this central project were generated, in order of priority: (1) growth on glucose (n= 30 samples) (2) growth on ethanol (n= 21) and (3) pheromone effect (n= 51 samples; combined glucose and ethanol samples). For each sample, every omic type was analysed (n=4; transcriptomic, proteomic, phosphoproteomic, and metabolomic). Total omic samples generated for project = 204.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKate Campbell
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList6x(13)C labeled residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-29 01:08:50ID requested
12020-03-26 01:59:24announced
22020-03-30 01:19:05announced2020-03-30: Updated publication reference for PubMed record(s): 32213592.
Publication List
Campbell K, Westholm J, Kasvandik S, Di Bartolomeo F, Mormino M, Nielsen J, Building blocks are synthesized on demand during the yeast cell cycle. Proc Natl Acad Sci U S A, 117(14):7575-7583(2020) [pubmed]
Keyword List
submitter keyword: Budding yeast, cell cycle, mitosis, fermentation, respiration, SILCA, iBAQ, LC-MSMS, multi-omics
Contact List
Jens B Nielsen
contact affiliationJens Nielsen, PhD, Dr. Tech. Professor, CEO of BII Systems and Synthetic Biology Chalmers University of Technology Kemivägen 10 SE-412 96 Göteborg SWEDEN BioInnovation Institute Ole Maaløes Vej 3 DK 2200 København N DENMARK
contact emailnielsenj@chalmers.se
lab head
Kate Campbell
contact affiliationChalmers University of Technology
contact emailkatec@chalmers.se
dataset submitter
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