PXD016519

PXD016519 is an original dataset announced via ProteomeXchange.

Title	Yeast cell cycle proteome and phosphoproteome (LC-MSMS)
Description	Multi-omic absolute quantitative analysis of the yeast (Saccharomyces cerevisiae) mitotic cell cycle. Transcriptomics (RNA-Seq), proteomics (SILAC/ iBAQ), phosphoproteomics (SILAC/ iBAQ, enrichment with TiO2), and untargeted metabolomics (Metabolon, Inc.) were performed, all in biological triplicate. Three sub-projects from this central project were generated, in order of priority: (1) growth on glucose (n= 30 samples) (2) growth on ethanol (n= 21) and (3) pheromone effect (n= 51 samples; combined glucose and ethanol samples). For each sample, every omic type was analysed (n=4; transcriptomic, proteomic, phosphoproteomic, and metabolomic). Total omic samples generated for project = 204.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:31.211.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kate Campbell
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-29 01:08:50	ID requested
1	2020-03-26 01:59:24	announced
2	2020-03-30 01:19:05	announced	2020-03-30: Updated publication reference for PubMed record(s): 32213592.
⏵ 3	2024-10-22 05:01:33	announced	2024-10-22: Updated project metadata.

Campbell K, Westholm J, Kasvandik S, Di Bartolomeo F, Mormino M, Nielsen J, Building blocks are synthesized on demand during the yeast cell cycle. Proc Natl Acad Sci U S A, 117(14):7575-7583(2020) [pubmed]

10.1073/pnas.1919535117;

submitter keyword: LC-MSMS,Budding yeast, multi-omics, mitosis, respiration, fermentation, iBAQ, cell cycle, SILCA

Jens B Nielsen
contact affiliation	Jens Nielsen, PhD, Dr. Tech. Professor, CEO of BII Systems and Synthetic Biology Chalmers University of Technology Kemivägen 10 SE-412 96 Göteborg SWEDEN BioInnovation Institute Ole Maaløes Vej 3 DK 2200 København N DENMARK
contact email	nielsenj@chalmers.se
lab head
Kate Campbell
contact affiliation	Chalmers University of Technology
contact email	katec@chalmers.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016519

PRIDE project URI

• PRIDE
  1. PXD016519
     1. Label: PRIDE project
     2. Name: Yeast cell cycle proteome and phosphoproteome (LC-MSMS)