PXD016508
PXD016508 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex |
Description | A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines. ADAM10 was recently proposed to function as six distinct scissors with different substrates, depending on its association with one of six regulatory tetraspanins, termed TspanC8s. However, it remains unclear whether ADAM10 function is critically dependent on a TspanC8 partner. To address this, we generated the first monoclonal antibodies to Tspan15 as a model TspanC8. These were used to show that ADAM10 is the principal Tspan15-interacting protein, that Tspan15 expression requires ADAM10 in cell lines and primary cells, and that a synthetic ADAM10/Tspan15 fusion protein is a functional scissor. Together these findings suggest that ADAM10 exists as an ADAM10/TspanC8 scissor complex. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-24 |
AnnouncementXML | Submission_2021-08-24_03:09:15.180.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephan Mueller |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-11-28 05:09:17 | ID requested | |
⏵ 1 | 2021-08-24 03:09:15 | announced |
Publication List
Koo CZ, Harrison N, Noy PJ, Szyroka J, Matthews AL, Hsia HE, M, ü, ller SA, T, ü, shaus J, Goulding J, Willis K, Apicella C, Cragoe B, Davis E, Keles M, Malinova A, McFarlane TA, Morrison PR, Nguyen HTH, Sykes MC, Ahmed H, Di Maio A, Seipold L, Saftig P, Cull E, Pliotas C, Rubinstein E, Poulter NS, Briddon SJ, Holliday ND, Lichtenthaler SF, Tomlinson MG, The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex. J Biol Chem, 295(36):12822-12839(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: TSPAN15, ADAM10 |
Contact List
Stefan F. Lichtenthaler | |
---|---|
contact affiliation | DZNE Munich Neuroproteomics Feodor-Lynen Str. 17 81377 Munich Germany |
contact email | stefan.lichtenthaler@dzne.de |
lab head | |
Stephan Mueller | |
contact affiliation | DZNE Munich Neuroproteomics |
contact email | stephan.mueller@dzne.de |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD016508 |
PRIDE project URI |
Repository Record List
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