⮝ Full datasets listing
PXD016506
PXD016506 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiling identifies novel targets modulated by the repurposed drug candidate nitroxoline in AsPC-1 pancreatic cancer cell line |
| Description | Protein expression levels after 24h and 48h treatment with nitroxoline in AsPC-1 cells were measured using a label free shotgun proteomics approach on a Bruker maXis mass spectrometer. Proteins were extracted and solubilized in Urea Tris buffer and quantified according to the Bradford assay. 50 ug of proteins were loaded on a 10 kDa cutoff centrifugal filter and trypsin digested according to the FASP protocol. Biological and technical triplicates were acquired for each condition. Raw data were processed using PEAKS Studio 7.5 (Bioinformatics Solutions Inc.) and searched using the PEAKS search engine against neXtProt database (June 2017; 42,151 total entries). Parent Mass Error Tolerance was set to 10.0 ppm and Fragment Mass Error Tolerance to 0.05 Da. Other search parameters were trypsin enzyme specificity, two missed cleavages per peptide, fixed Carbamidomethylation of Cys and variable Oxidation of Met, with two variable PTM per peptide. Non-specific cleavage was allowed to both ends of peptides. FDR estimation was enabled, and precursor options corrected. The FDR threshold at PSMs level was set to 0.5%, resulting in FDR at peptide and protein level lower than 1.0%. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-20 |
| AnnouncementXML | Submission_2026-04-20_00:38:46.603.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Maurizio Ronci |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | maXis |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-11-28 04:16:19 | ID requested | |
| ⏵ 1 | 2026-04-20 00:38:47 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Nitroxoline, Pancreatic Cancer, label-free shotgun proteomics, AsPC-1 |
Contact List
| Maurizio Ronci | |
|---|---|
| contact affiliation | University G. d'Annunzio |
| contact email | maurizio.ronci@unich.it |
| lab head | |
| Maurizio Ronci | |
| contact affiliation | University G. d'Annunzio ITALY |
| contact email | maurizio.ronci@unich.it |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v02/MSV000084631/ |
