PXD016502

PXD016502 is an original dataset announced via ProteomeXchange.

Title	Thiosulfate oxidation in Erythrobacter flavus 21-3 provides novel clues to the source of element sulfur in the deep-sea cold seep
Description	Zero-valent sulfur (ZVS) and thiosulfate are important intermediates in the biogeochemical cycle of sulfur. The former has been proved to be existed in the cold seep and hydrothermal systems. Three thiosulfate oxidation pathways are already identified and here we found a novel one in Erythrobacter, which was the first genus in the family Erythrobacteraceae that could accumulate ZVS during thiosulfate oxidation. Erythrobacter flavus 21-3 was isolated from the sediment of clod seep. Genomic analyses showed the typical genes encoding Sox multienzyme complex were absent in the genome of E. flavus 21-3. Through proteome and genome data, we identified a three gene involved pathway of thiosulfate oxidation, including thiosulfate dehydrogenase (tsdA), thiosulfohydrolase (soxB) and sulfur dioxygenases (sdo). For the first time, genetic operating system was constructed in Erythrobacter, and mutant strains ΔtsdA, ΔsoxB, ΔsdoA, ΔsdoB and ΔsdoAB were constructed. Stoichiometry was calculated and tetrathionate and ZVS were found to be the intermediates in this novel thiosulfate oxidation pathway. The diverse and distribution of these proteins were investigated and this novel thiosulfate oxidation pathway may exist in bacteria formerly ignored the function of ZVS production. The results give a powerful evidence for a new source of biogenetic ZVS in cold seep.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_17:11:01.764.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sun lab sun lab
SpeciesList	scientific name: Erythrobacter flavus; NCBI TaxID: 192812;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-28 03:19:52	ID requested
⏵ 1	2021-09-08 17:11:02	announced

Zhang J, Liu R, Xi S, Cai R, Zhang X, Sun C, A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea. ISME J, 14(9):2261-2274(2020) [pubmed]

submitter keyword: Zero-valent sulfur (ZVS), thiosulfate, Erythrobacter, cold seep, bacteria

Chaomin Sun
contact affiliation	Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
contact email	sunchaomin@qdio.ac.cn
lab head
sun lab sun lab
contact affiliation	institute of oceanology
contact email	1365105894@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016502
     1. Label: PRIDE project
     2. Name: Thiosulfate oxidation in Erythrobacter flavus 21-3 provides novel clues to the source of element sulfur in the deep-sea cold seep