PXD016495

PXD016495 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics on colorectal cancer MCTS upon OXPHOS inhibition
Description	Previous high throughput gene expression profiling combined with a HCT116 colon cancer tumor spheroid-based drug-screening assay identified context-dependent treatment responses to OXPHOS inhibitors resulting in a shunting towards the mevalonate pathway. To complement these findings with a characterization of the proteome we use mass-spectrometry based proteomics to investigate protein profiles and context-dependent treatment responses in PMCTS and QMCTS compared to monolayer cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:10:04.192.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Steinmetz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-28 03:14:36	ID requested
1	2020-07-28 18:31:04	announced
⏵ 2	2024-10-22 05:10:12	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acsomega.0c01419;
Steinmetz J, Senkowski W, Lengqvist J, Rubin J, Ossipova E, Herman S, Larsson R, Jakobsson PJ, Frykn, ä, s M, Kultima K, Descriptive Proteome Analysis to Investigate Context-Dependent Treatment Responses to OXPHOS Inhibition in Colon Carcinoma Cells Grown as Monolayer and Multicellular Tumor Spheroids. ACS Omega, 5(28):17242-17254(2020) [pubmed]

10.1021/acsomega.0c01419;

Steinmetz J, Senkowski W, Lengqvist J, Rubin J, Ossipova E, Herman S, Larsson R, Jakobsson PJ, Frykn, ä, s M, Kultima K, Descriptive Proteome Analysis to Investigate Context-Dependent Treatment Responses to OXPHOS Inhibition in Colon Carcinoma Cells Grown as Monolayer and Multicellular Tumor Spheroids. ACS Omega, 5(28):17242-17254(2020) [pubmed]

Keyword List

submitter keyword: colon cancer, OXPHOS,Proteomics, spheroids, energy metabolism

submitter keyword: colon cancer, OXPHOS,Proteomics, spheroids, energy metabolism

Contact List

Kim Kultima
contact affiliation	Department of Medical Sciences, Clinical Chemistry, Uppsala University, Sweden
contact email	kim.kultima@medsci.uu.se
lab head
Julia Steinmetz
contact affiliation	Karolinska Institutet
contact email	julia.steinmetz@ki.se
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016495
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016495

PRIDE project URI

Repository Record List

[ + ]