PXD016484
PXD016484 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Concentration determination of >200 proteins in dried blood spots for biomarker discovery and validation |
| Description | Raw files were created using XCalibur 3.0.63 (Thermo Scientific) software and analysed with Thermo Proteome Discoverer 2.2.0.388 software suite (Thermo Scientific). The raw data files were processed as previously described 26 with the following modifications: the peak lists were submitted to Mascot 2.4.1and Sequest servers using the UniProt human database 28 which contained 20,338 protein sequences. The enzyme was set to Trypsin (Full) with max missed cleavage set to 2. The fixed modification was set to carbamidomethyl and variable modifications were set as oxidation (M), deamidation (NQ), and N-terminal methionine loss. Precursor and fragment mass tolerances were set at 8 ppm and 0.8 Da, respectively. Peptide spectral match validation was performed using Percolator setting the maximum Delta Cn to 0.05 and decoy database searches at 0.01 for target FDR (Strict), 0.05 (relaxed) and validation based on q-Value |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-01-06 |
| AnnouncementXML | Submission_2020-01-06_10:35:07.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | azad eshghi |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-11-27 09:57:39 | ID requested | |
| ⏵ 1 | 2020-01-06 10:35:08 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: dired blood spot |
Contact List
| Christoph H. Borchers | |
|---|---|
| contact affiliation | Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Quebec, H3T 1E2, Canada |
| contact email | christoph.borchers@mcgill.ca |
| lab head | |
| azad eshghi | |
| contact affiliation | Uvic GBC PC |
| contact email | azad@proteincentre.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084629/ |
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