PXD016481

PXD016481 is an original dataset announced via ProteomeXchange.

Title	Bone morphogenetic protein 4 in human saliva during orthodontic therapy
Description	In orthodontic therapy periodontal tissue responds to mechanical stimuli by bone remodeling mediated by specific molecules involved in periodontal regeneration and homeostasis. The resulting changes are reflected in the salivary proteome that is emerging as a valuable diagnostic tool. We analyzed the changes of saliva proteome during orthodontic tooth movement in 12 healthy male patients, presented with malocclusion treated by placement of fixed orthodontic appliance. Six patients with identical dental pathology, but no orthodontic therapy were used as control samples. Saliva was collected a day before, immediately after, and 2, 7 and 30 days after the placement of the fixed orthodontic appliance and analyzed by LC-MS. Total of 198 proteins were identified and classified on the basis of their functional characteristics. Proteins involved in bone remodeling, inflammation and healing were detected mostly 30 days after placement of the fixed orthodontic appliance. At this time point, Bone morphogenetic protein 4 emerged as a central player in the ongoing dental bone remodeling. Besides BMP4, BMP antagonists: BMP Binding Endothelial Regulator (BMPER), Insulin-like growth factor-binding protein 3 (IGFBP3), Cytoskeleton-associated protein 4 (CKAP4) and Fibroblast growth factor 5 (FGF5), were also identified. Presence of BMP4 in human saliva 30 days after the placement of orthodontic fixed appliance was confirmed by ELISA and concentration of BMP4 was 3.2 pg/ml. To date, there is no published data on the presence of BMP molecules or their antagonists in saliva or the gingival cervical fluid, regardless of different orthodontic treatments or disease. BMP4 is involved not only in orthodontic treatment, but also in processes involving tooth development and homeostasis. Therefore, a better understanding of its networks in bone remodeling during OTM is heralded.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:04.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruđer Novak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-27 05:41:06	ID requested
1	2021-09-08 17:13:07	announced
⏵ 2	2024-10-22 05:27:05	announced	2024-10-22: Updated project metadata.

Grgurevic L, Novak R, Salai G, Trkulja V, Hamzic LF, Popovic VZ, Bozic D, Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance. Prog Orthod, 22(1):19(2021) [pubmed]

10.1186/s40510-021-00364-6;

submitter keyword: saliva, proteomics, BMPER, bone remodeling, orthodontic tooth movement, BMP4

Lovorka Grgurević
contact affiliation	Center for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia Drago Perović Department of Anatomy, School of Medicine, University of Zagreb, Zagreb, Croatia
contact email	lovorka.grgurevic@mef.hr
lab head
Ruđer Novak
contact affiliation	School of Medicine, University of Zagreb
contact email	rudjer.novak@mef.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD016481

PRIDE project URI

• PRIDE
  1. PXD016481
     1. Label: PRIDE project
     2. Name: Bone morphogenetic protein 4 in human saliva during orthodontic therapy