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PXD016481

PXD016481 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBone morphogenetic protein 4 in human saliva during orthodontic therapy
DescriptionIn orthodontic therapy periodontal tissue responds to mechanical stimuli by bone remodeling mediated by specific molecules involved in periodontal regeneration and homeostasis. The resulting changes are reflected in the salivary proteome that is emerging as a valuable diagnostic tool. We analyzed the changes of saliva proteome during orthodontic tooth movement in 12 healthy male patients, presented with malocclusion treated by placement of fixed orthodontic appliance. Six patients with identical dental pathology, but no orthodontic therapy were used as control samples. Saliva was collected a day before, immediately after, and 2, 7 and 30 days after the placement of the fixed orthodontic appliance and analyzed by LC-MS. Total of 198 proteins were identified and classified on the basis of their functional characteristics. Proteins involved in bone remodeling, inflammation and healing were detected mostly 30 days after placement of the fixed orthodontic appliance. At this time point, Bone morphogenetic protein 4 emerged as a central player in the ongoing dental bone remodeling. Besides BMP4, BMP antagonists: BMP Binding Endothelial Regulator (BMPER), Insulin-like growth factor-binding protein 3 (IGFBP3), Cytoskeleton-associated protein 4 (CKAP4) and Fibroblast growth factor 5 (FGF5), were also identified. Presence of BMP4 in human saliva 30 days after the placement of orthodontic fixed appliance was confirmed by ELISA and concentration of BMP4 was 3.2 pg/ml. To date, there is no published data on the presence of BMP molecules or their antagonists in saliva or the gingival cervical fluid, regardless of different orthodontic treatments or disease. BMP4 is involved not only in orthodontic treatment, but also in processes involving tooth development and homeostasis. Therefore, a better understanding of its networks in bone remodeling during OTM is heralded.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:27:04.976.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuđer Novak
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Discovery
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-27 05:41:06ID requested
12021-09-08 17:13:07announced
22024-10-22 05:27:05announced2024-10-22: Updated project metadata.
Publication List
Grgurevic L, Novak R, Salai G, Trkulja V, Hamzic LF, Popovic VZ, Bozic D, Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance. Prog Orthod, 22(1):19(2021) [pubmed]
10.1186/s40510-021-00364-6;
Keyword List
submitter keyword: saliva, proteomics, BMPER, bone remodeling, orthodontic tooth movement, BMP4
Contact List
Lovorka Grgurević
contact affiliationCenter for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia Drago Perović Department of Anatomy, School of Medicine, University of Zagreb, Zagreb, Croatia
contact emaillovorka.grgurevic@mef.hr
lab head
Ruđer Novak
contact affiliationSchool of Medicine, University of Zagreb
contact emailrudjer.novak@mef.hr
dataset submitter
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