PXD016478 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Swiprosin1 controls cargo-specific entry to clathrin and dynamin-independent endocytic pathway |
Description | The clathrin and dynamin-independent endocytic pathway (CLIC-GEEC) is the major route of internalization of a large fraction of glycosylphosphatidylinositol-anchored proteins (GPI-APs) and other cell surface receptors. Unlike the clathrin-dependent endocytic pathway, where adaptors recruited to the endocytic pit provide the clues to recruit specific cargo, the CLIC-GEEC pathway is considered to take up portions of the membrane without any specificity. Here, we identified actin-binding protein swiprosin1 as the first cargo adaptor for the CLIC-GEEC pathway. We found that swiprosin1 interacts with active Rab21, a known regulator of integrin endocytosis, and couples Rab21 and the integrin cargo to the CLIC-GEEC endocytic machinery. Our work also identified the previously unknown pathway by which Rab21 mediates integrin endocytosis and linked the CLIC-GEEC pathway to integrin-dependent cancer cell invasion. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-18 |
AnnouncementXML | Submission_2021-08-18_05:02:29.230.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Henrik Zauber |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-27 05:40:02 | ID requested | |
⏵ 1 | 2021-08-18 05:02:29 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Endocytosis,Rab21,Clathrin-independent,Swiprosin,Human,SILAC,co-IP |
Contact List
Matthias Selbach |
contact affiliation | Max Delbrück Center for Molecular Medicine in the Helmholtz Association Robert-Rössle-Straße 10 13125 Berlin-Buch |
contact email | matthias.selbach@mdc-berlin.de |
lab head | |
Henrik Zauber |
contact affiliation | MDC Berlin-Buch |
contact email | henrik.zauber@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016478
- Label: PRIDE project
- Name: Swiprosin1 controls cargo-specific entry to clathrin and dynamin-independent endocytic pathway