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PXD016478

PXD016478 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSwiprosin1 controls cargo-specific entry to clathrin and dynamin-independent endocytic pathway
DescriptionThe clathrin and dynamin-independent endocytic pathway (CLIC-GEEC) is the major route of internalization of a large fraction of glycosylphosphatidylinositol-anchored proteins (GPI-APs) and other cell surface receptors. Unlike the clathrin-dependent endocytic pathway, where adaptors recruited to the endocytic pit provide the clues to recruit specific cargo, the CLIC-GEEC pathway is considered to take up portions of the membrane without any specificity. Here, we identified actin-binding protein swiprosin1 as the first cargo adaptor for the CLIC-GEEC pathway. We found that swiprosin1 interacts with active Rab21, a known regulator of integrin endocytosis, and couples Rab21 and the integrin cargo to the CLIC-GEEC endocytic machinery. Our work also identified the previously unknown pathway by which Rab21 mediates integrin endocytosis and linked the CLIC-GEEC pathway to integrin-dependent cancer cell invasion.
HostingRepositoryPRIDE
AnnounceDate2021-08-18
AnnouncementXMLSubmission_2021-08-18_05:02:29.230.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHenrik Zauber
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-27 05:40:02ID requested
12021-08-18 05:02:29announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Endocytosis,Rab21,Clathrin-independent,Swiprosin,Human,SILAC,co-IP
Contact List
Matthias Selbach
contact affiliationMax Delbrück Center for Molecular Medicine in the Helmholtz Association Robert-Rössle-Straße 10 13125 Berlin-Buch
contact emailmatthias.selbach@mdc-berlin.de
lab head
Henrik Zauber
contact affiliationMDC Berlin-Buch
contact emailhenrik.zauber@mdc-berlin.de
dataset submitter
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Dataset FTP location
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